Methanolic Root Extract of Rauwolfia serpentina Improves the Glucose Tolerance in Wister Mice

Rauwolfia serpentina根甲醇萃取物增強小鼠葡萄糖耐受量

Muhammad Bilal Azmi ； Shamim A. Qureshi

《藥物食品分析》 20卷2期 (2012/06) Pp. 484-488

https://doi.org/10.6227/jfda.2012200208

並列摘要

In the present study, acute toxicity of methanolic root extract (MREt) of Rauwolfia serpentina (10 - 250 mg/kg) was observed in terms of hypoglycemic activity and behavior pattern including sedation and mortality rate in different groups of normoglycemic wister mice (6/group). Orally administrated MREt (10 to 60 mg/kg) was found non-sedative and showed hypoglycemic activity by reducing the mean blood glucose level from 80 to 62 mg/dL after 30 min of administration while the doses (100-250 mg/kg) of same extract showed sedation and mortality rate from 17 to 100% within 4 h. The value of median lethal dose (LD50) of MREt of R. serpentina was 141.25 mg/kg (log LD50 = 2.15 mg/kg) from graph plotted between log-doses verses probits. In oral glucose tolerance test, MREt (10, 30 and 60 mg/kg) showed significant hypoglycemic activity at 0, 30 and 60 min by deceasing blood glucose level ranging from 31- 65%, 39 - 49% and 25 - 51% respectively compared to control and negative control (treated with 0.05% dimethylsulfoxide @ 1 mL/kg) groups. However, MREt (100 mg/kg), though hypoglycemic from 0 to 120 min, induced slight sedation and mortality in experimental mice. The results indicated that the MREt (10 - 60 mg/kg) was non-sedative and effective in lowering the blood glucose level in mice but showed lethal effect in terms of sedation and mortality at doses ≥ 100 mg/kg. Therefore, MREt of R. serpentina in small doses can improve the glucose tolerance either at pancreatic or extra-pancreatic level.

並列關鍵字

acute toxicity ； hypoglycaemic activity ； LD50 ； oral glucose tolerance test ； Rauwolfia serpentina

參考文獻

Rotimi, S. O., Omotosho, O. E. and Rotimi, O. A. 2011. Persistence of acidosis in alloxan- induced diabetic rats treated with the juice of Asytasia gangetica leaves. Pharmacogn. Mag. 7: 25-30.

Wild, S., Roglic, G., Green, A., Sicree, R. and King, H. 2004. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 27: 1047-1053.

Sinha, A., Hoerger, T., Rajan, M. and Pogach, L. 2010. Costs and consequences associated with newer medications for glycemic control in type 2 diabetes. Diabetes Care 33: 695-700.

Shukia, R., Sharma, S. B., Puri, D., Prabhu, K. M. and Murthy, P. S. 2000. Medicinal plants for treatment of diabetes mellitus. Ind. J. Clinical Biochem. (Suppl.) 15: 169-177.

Malviya, N., Jain, S. and Malviya, S. 2010. Antidiabetic potential of medicinal plants. Acta Pol. Pharm. 67: 113-118.