Bacillus mycoides CHT2402對萵苣幼苗生長之影響

Effect of Bacillus mycoides on Seedlings Growth of Lettuce

陳和緯(H. W. Chen) ； 林盈宏(Y. H. Lin) ； 黃振文(J. W. Huang) ； 張碧芳(P. F. L. Chang)

《植物病理學會刊》 19卷2期 (2010/06) Pp. 157-165

https://doi.org/10.6649/PPB.201006_19(2).0005

摘要

植物根圈促生細菌(Plant growth-promoting rhizobacteria, PGPR)可分泌植物生長的調控因子，如植物荷爾蒙，來活化根部的代謝作用，或是藉由分泌抗生物質、嵌鐵物質、競爭生態位與養份及誘導植株抗性等作用來避免或降低植物遭受病原菌侵害之機率，進而維持或促進植物生長。本研究中發現蕈狀芽孢桿菌Bacillus mycoides (BM) CHT2402具有産生吲哚乙酸(indole-3-acetic acid, IAA)與氨(NH3)之特性，若施用BM CHT2402菌株於不同作物上觀察其促進作物生長的能力，我們發現栽植番茄、西瓜及萵苣植株於BM CHT2402的細菌懸浮液混拌介質中三週後，可增加植株鮮重，且其中萵苣明豐三號品種效果最佳。但若嘗試以澆灌法施用BM CHT2402細菌縣浮液於番茄、西瓜及萵苣植株時，此促生細菌則無促進植物生長之效果。此外若將萵苣三品種種子播種於BM CHT2402混拌介質中，萵苣植株的生長明顯提高，但促進效果卻因不同品種而有所差異。

關鍵字

植物根圈促生細菌 ； 蕈狀芽孢桿菌 ； 萵苣

並列摘要

Plant growth-promoting rhizobacteria could enhance plant growth by providing plant growth regulators such as phytohormones to activate the metabolic activities of plant roots, so as to protect plants from attack by phytopathogens or reduce the disease severity by secreting antibiotics or siderphores, competing for the ecological niches or nutrition, and inducing plant defense responses. In this study, Bacillus mycoides (BM) CHT2402 was used to test its ability on growth promotion of different crops. The abilities of BM CHT2402 to produce indole-3-acetic acid (IAA) and ammonia (NH3) were observed. Media mixed with cell suspensions of BM CHT2402 increased the fresh weight of tomato, watermelon, and lettuce plants, especially on Ming-feng NO.3 cultivar of lettuce. Media irrigated with cell suspensions of BM CHT2402, however, had no significant growth-promoting effects on these plants. Media mixed with cell suspensions of BM CHT2402 applied on three different lettuce cultivars could promote lettuce growth, but different growth-promoting effects were observed depending on the cultivars tested.

並列關鍵字

plant growth-promoting rhizobacteria ； Bacillus mycoides ； lettuce