The Clinical Practice Guideline (CPG) must be reviewed through rigorous and systematic method in order to promote the maximum effectiveness of clinical services and also reduce the variation of the practices. The main purpose of this paper is to provide the better quality of CPG for optimizing patient care services. The application of CPG to optimize nursing interventions and enhance the patient's prognostic potential is an essential ability of nursing staff. This article will introduce two frequently applied clinical care guidelines application models, including the knowledge to action framework and the CAN-implement framework, with two examples as rationales. At the same time, distinctive suggestions for the application of clinical care guidelines are also cited from the perspective of non-medical professionals.