成功由TNG66甘諸中萃取β-carotene,使用溶劑為acetone溶液,萃取溫度為室溫(25℃),acetone的濃度建議高於75%;萃取製程含有兩個變數分別是acetone濃度與萃取時間,根據反應曲面(RSM)的實驗設計法,實驗結果顯示acetone濃度對萃取液的吸光值有顯著的影響,而建議的最適化萃取條件為90% acetone與萃取時間30min; 和單次萃取相比,多次萃取可以提高β-carotene的萃取量,雖然多次萃取可以將萃取率提升到80%以上,但萃取次數的選擇必須搭配經濟效益分析而決定。TNG66甘諸含有β-carotene及其他的carotene類似物,但以all-trans β-carotene為最多。
β-carotene was successfully extracted from TNG66 sweet potatoes using acetone as the extraction solvent. The extraction temperature was 25℃. The most effective acetone concentration was higher than 75%. Two variables, acetone concentration and extraction time, were considered as major parameters of the β-carotene extraction process. Based on response surface methodology (RSM) results, the absorbance of the β-carotene extraction solution was significantly affected by the acetone concentration. The optimal operating conditions were the use of 90% acetone and an extraction time of 30 minutes. Increasing the extraction number improved the total amount of extracted β-carotene. Although the β-carotene extraction rate could be increased to above 80% after multiple extractions, the determination of the extraction number should be compensated with the result of economical analysis. TNG66 sweet potatoes contain significant amounts of β-carotene and carotene derivatives. The major component was the all-trans β-carotene.