- 期刊
- OpenAccess
Effect of Lactoferrin and Its Hydrolysates Prepared with Pepsin and Trypsin on Escherichia coli O157:H7
摘要
As A Glycoprotein, Lactoferrin (LF) has many biological actions, among which the antibacterial activity is one of the most important effects studied. This study detected the antibacterial activity of bovine LF against Escherichia coli O157:H7. The LF hydrolysate (LFH) was acquired with trypsin and pepsin. It was found that the hydrolysates produced with each inhibited bacterial growth although the activities of trypsin hydrolysate were weaker than those of pepsin hydrolysate. Antibacterial activities of all hydrolysates were corresponding to the fraction with a molecular mass greater than 6kDa. Sulfonic acid derivatives were utilized for fractionation of peptides in hydrolysates and the cationic peptides were investigated employing mass spectrometry.
延伸閱讀
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