Objective: Investigate the results of bacterial cultures from patients with appendicitis to aid in the choice of antibiotics. Methods: Retrospectively examine bacterial cultures from 308 cases of pathologically proved appendicitis and record the bacterial species. Results: Peritoneal/wound cultures showed positive results in 83.9% of 149 patients. Blood cultures showed positive results in 16.1% of 236 patients. The five most common bacterial species in both blood and peritoneal/wound cultures were E coli, Bacteroides sp., Streptococci, Pseudomonas sp., and Klebsiella sp. Gram-negative bacteria were the most common. Sixty eight percent of patients had mixed types of bacteria. Conclusion: Bacteria were cultured in a high percentage of peritoneal/wound cultures. More than two-third of patients has two or more types of bacteria. Empiric antibiotics should cover Gram-negative and anaerobic bacteria.