- 期刊
鼠李糖乳桿菌（Lacticaseibacillus rhamnosus）GKY7具有改善酒精性脂肪肝的潛在效益
Potential Effect of Probiotic Lacticaseiballus rhamnosus GKY7 on Alleviating Alcoholic Fatty Liver
摘要
引起酒精性肝病（alcoholic liver disease, ALD）的主要原因為長期過量飲酒，酒精在肝臟中經由酒精脫氫酶（alcohol dehydrogenase）代謝成乙醛（acetaldehyde），肝臟中的乙醛分解不足將導致脂肪堆積形成脂肪肝（fatty liver），從而增加患ALD、肝硬化（cirrhosis）、甚至肝癌（liver cancer）的風險。甚至肝癌的發生。本研究調查五種不同益生菌菌株，包括Lacticaseibacillus rhamnosus（鼠李糖乳桿菌）GKY7, Pediococcus acidilactici（酸化小球菌）GKA4, Lactiplantibacillus plantarum（植物乳桿菌）GKK1, Limosilactobacillus reuteri（利莫西林乳桿菌）GKR1與Pediococcus pentosaceus（五味子小球菌）GKP4對降低ALD風險的潛在效益。總共60隻C57BL/6小鼠被分為正常組（未處理）、酒精飲食組和酒精飲食＋益生菌組，每組5隻小鼠。以酒精液態飼料（Lieber-DeCarli ethanol diet, LDE）餵養6週，誘導酒精飲食組和酒精飲食＋益生菌組的小鼠發展為酒精性脂肪肝。飲食＋益生菌組的小鼠同時餵食五種不同的益生菌中的一種，益生菌粉分為兩個不同劑量（3.0×10^(11) CFU/g），低劑量組的小鼠每天餵食的劑量相當於60公斤成人攝食的0.1克，而高劑量組則相當於0.5克。六週後，餵食益生菌組的小鼠血清中丙胺酸轉胺酵素（alanine transaminase, AST）和天門冬胺酸轉胺酶（aspartate transaminase, ALT）均顯著低於僅餵食LDE的小鼠。在測試的5種不同的益生菌菌株中，Lacticaseibacillus rhamnosus GKY7效果最為顯著。此外，菌株GKY7亦可降低血清中的三酸甘油酯（triglycerides, TG）與總膽固醇（total cholesterol, TC）含量，並且增加肝臟中的酵素榖胱甘肽還原酶（glutathione reductase, GRd）、超氧化物歧化酶（superoxide dismutase, SOD）含量以及榖胱甘肽過氧化物酶（glutathione peroxidase, GPx）的活性，這些結果表明，益生菌L. rhamonsus GKY7具有降低ALD風險的潛力。由於低劑量和高劑量之間的效果沒有明顯差異，吾等建議60公斤的成年人每天攝入0.1克L. rhamonsus GKY7以預防ALD的發生。
並列摘要
A major cause of alcoholic liver disease (ALD) is long-term excessive consumption of alcohol, which is metabolized by alcohol de-hydrogenase in the liver to acetaldehyde. Inadequate break down of acetaldehyde may result in fat accumulation to form fatty liver, thus increasing the risk of developing ALD, liver cirrhosis, or even liver cancer. In this study, we investigated the potential effect of five different strains of probiotics on reducing the risk of ALD, including Lacticaseibacillus rhamnosus GKY7, Pediococcus acidilactici GKA4, Lactiplantibacillus plantarum GKK1, Limosilactobacillus reuteri GKR1, and Pediococcus pentosaceus GKP4. A total of 60 C57BL/6 mice were divided into normal (untreated), alcohol diet, and alcohol diet + probiotics groups with 5 mice in each group. Mice in alcohol diet and alcohol diet + probiotics groups were induced to develop alcoholic fatty liver by feeding with Lieber-DeCarli ethanol diet (LDE) for 6 weeks. Mice in the diet + probiotics group were si multaneously fed with one of the five different probiotics at two different doses of probiotic powder (3.0×10^(11) CFU/g). Mice in the low dose group were given a daily dose equivalent to 0.1 g, and those in the high dose group were given a daily dose equivalent to 0.5 g for a 60-kg adult. Compared to untreated mice, the serum levels of alanine transaminase and aspartate transaminase of treated mice were significantly lower. Among the 5 different strains of probiotics tested, Lacticaseibacillus rhamnosus GKY7 showed the most significant effects. In addition, strain GKY7 was also found to reduce the levels of triglycerides and total cholesterol in serum and liver, and increase the levels of glutathione reductase and superoxide dismutase and the activity of glutathione peroxidase in the liver. These results suggest that the probiotic L. rhamnosus GKY7 has the potential to reduce the risk of ALD. As there was no significant difference in effect between low and high doses, we recommend a daily intake of 0.1 g of L. rhamnosus GKY7 for a 60-kg adult to prevent the development of ALD.