台灣朝鮮薊樣品生物活性成份分析

Bioactive Ingredient Analysis of Taiwan Artichoke

顏嘉勁(Jia-jin Yan)

指導教授 ： 曾惠芬

國立暨南國際大學/科技學院/應用化學學系/碩士(2017年)

摘要

朝鮮薊(Artichoke, Cynara scolymus L.)原產於地中海沿岸，現今被世界各國廣泛種植。近年來國外朝鮮薊定性定量研究顯示其主要成分由咖啡奎寧酸、多酚類和黃酮類化合物組成，在功能方面國外研究已證實具有保肝、抗癌、抗氧化、降膽固醇的作用。近幾年天健生物科技公司引進朝鮮薊在台灣種植，所以本研究針對各式台灣朝鮮薊的樣品分別進行熱水萃取與酒精萃取，再以HPLC配合UV偵測器或質譜儀進行生物活性成份分析。實驗結果顯示：種植地的氣候或處理過程對朝鮮薊莖葉生物活性成份含量有所影響，木犀草苷僅存在於朝鮮薊葉的相關產品，如膠囊與凍晶粉中；相反地，朝鮮薊葉柄則含較高的綠原酸與洋薊酸。另外，γ-ray照射有助於生物活性成份的釋出；而且低溫烘焙對各主要抗氧化成分的保存較佳。

關鍵字

朝鮮薊 ； HPLC ； 成份分析

並列摘要

Artichoke ( Cynara scolymus L.) is indigenous to the coast of Mediterranean , but has become widely naturalized around the world. Qualitative and quantitative study of artichoke suggests that its major components are caffeoylquinic acid‚ polyphenols and flavonoids. In terms of functionality studies‚ it has confirmed effects on hepatoprotection, anticancer, antioxidation, and cholesterol-lowering. In recent years, Tianjian biotechnology company introduces artichoke cultivation in Taiwan‚ so we studied various samples of Taiwan artichoke. Followed by hot water extraction and alcohol extraction‚ bioactive ingredient analysis was performed by HPLC coupled UV detector or mass spectrometer. The results show that planting season and the process of the raw material have significant impacts on bioactive ingredients in artichoke. Luteolin exists more in artichoke leaf related products‚ such as capsules and frozen crystalline powder; on the contrary‚ artichoke leafstalk contains a higher content of chlorogenic acid and cynarin. In addition‚ γ-ray irradiation helps release bioactive ingredients; low temperature baking is better for preservation of major antioxidants.

並列關鍵字

Artichoke ； HPLC ； Bioactive ingredients analysis

參考文獻

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