Effects of Crude Adlay Hull Acetone Extract on Steroidogenesis from Rat Zona Fasciculata-Reticularis Cells

薏苡殼丙酮粗萃取物對大鼠束網狀細胞類固醇生合成的效應

張玲玲(L. L. Chang)

《華岡工程學報》 25期 (2010/01) Pp. 77-83

https://doi.org/10.29987/CCUHKJE.201001.0011

摘要

在中國薏苡仁用於醫療及食物，它俱有抑制突變及降血脂的功能。本文主要是探討用丙酮萃取薏苡仁殼，其對大鼠腎上腺皮質束網狀細胞類固醇生合成的影響。由實驗發現薏苡仁殼萃取液，抑制大鼠腎上腺皮質束網狀細胞皮質脂酮的分泌。透過動力學分析，發現薏苡仁殼萃取液分別透過不競爭性壓抑和非競爭性壓抑，影響腎上腺皮質束網狀細胞類固醇生合成的第一及最後酵素的活性，藉以抑制皮質脂酮的分泌。

關鍵字

薏苡殼丙酮粗萃取物 ； 膽固醇解重碳酶 ； 11β-羥基化酶 ； 束網狀細胞

並列摘要

Adlay is a grass crop which has been used in traditional Chinese medicine and also as a nourishing food. It has been shown to posses anti-allergic, antimutagenic and hypolipemic effects. However, the steroidogenic effects of crude adlay hull acetone extract (AHA) on adrenal zona fasciculata-reticularis (ZFR) cells are still unclear. ZFR cells were incubated with AHA in the presence or absence of 25-hydroxy cholesterol (25-OH-cholesterol), pregnenolone, progesterone or deoxycorticosterone. The concentrations of corticosterone or pregnenolone in the media were measured by radioimmunoassay (RIA). The present data demonstrated that (1) AHA (800μg/ml) caused more pregnenolone release in control group, but not in 25-OH-cholesterol, trilostane or 25-OH-cholesterol+trilostane group; (2) kinetic study showed an uncompetitive inhibition model of AHA to P450 side chain cleavage enzyme (P450scc); (3) kinetic study showed a noncompetitive inhibition model of AHA to 11β-hydroxylase.

並列關鍵字

crude adlay hull acetone extract ； P450scc ； 11β-hydroxylase ； zona fasciculata-reticularis cells