Journal of International Dental and Medical Research
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Ectodermal Dysplasia Group-Turkey,正常發行
選擇卷期
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Previous studies demonstrated that fasting has beneficial effects on overall health, extending lifespan and that it rejuvenates cells, promoting tissue regeneration. Periodontitis is a prevalent oral inflammatory disease that leads to alveolar bone loss and linked to several systemic health problems. Ligature-induced periodontitis model is considered to be similar to human periodontitis in various aspect and is one of the most used animal models in periodontal research. The present study aims to investigate the effect of fasting regimen in experimentally-induced periodontitis in mice. In this study we applied two different kinds of fasting regimens: Intermittent fasting (IF / every other day fasting)and prolonged fasting (PF / 2 days fasting and 5 days free food intake) for 4 weeks. We found that both fasting regimen groups showed less amount of bone loss compared to the nonfasting groups at the ligature-periodontitis site, furthermore also at the contralateral side of the maxillae where the physiological bone loss occurs. PQCT and calcein-labeled histomorphometric analyses showed higher bone regeneration capacity in the fasting groups than in the nonfasting groups. Bone marrow cells from the femurs of the fasting groups produced more mineralized modules than the nonfasting groups. Data from this study showed fasting would be beneficial for resisting bone destruction in periodontitis and for maintaining periodontal bone health, which could be due to the increase of osteoprogenitor cells in bone marrow.
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The aim of this study was to evaluate the shear bond strengths of three types of metal brackets used in Indonesia. Forty human maxillary premolars, extracted for orthodontic purposes, were fixated in PVC cylinders using acrylic resins. The specimens were divided into four groups (n = 10) based on the type of bracket and adhesive used. The bond strengths of two types of Chinese metal brackets (Hangzhou 3B^(TM) and Zhejiang Protect^(TM)) bonded with Transbond XT^(TM) (3M, Unitek) and an American metal bracket (Mini Dyna Lock^(TM)) bonded with Transbond XT^(TM) and Enlight^(TM) (Ormco) were determined. Statistical analysis was conducted using independent samples t-test (p ＜ 0.05). The mean shear bond strengths of the 3B^(TM) and Protect^(TM) brackets were 6.09 ± 0.53 and 7.9 ± 0.61 MPa, respectively (p ＜ 0.05), and those of the Mini Dyna Lock^(TM) brackets using Transbond XT^(TM) and Enlight^(TM) were 12.46 ± 0.35 and 9.69 ± 0.53 MPa, respectively (p ＜ 0.05). These results suggested that the shear bond strengths of the Chinese metal brackets were within the acceptable range for orthodontic use. In addition, the American bracket bonded with Transbond XT^(TM) had a higher shear bond strength when compared with that bonded with Enlight^(TM).
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To evaluate the perceptions of dental students and interns about diagnosis and treatment of dentin hypersensitivity. In this cross-sectional study, participants were recruited from four dental colleges in Lahore, Pakistan. A self-administered validated questionnaire was used for data collection. Standard descriptive statistics were computed. Analytical statistical analysis was performed by using Pearson’s chi-square test. Mean age of participants was 23.48 ± 5.61 years. Most respondents 76.1% stated that they had seen patients with DH in the last 2-4 weeks. Eighty percent claimed that DH had an impact on the quality of life of patients while 64.7% thought that impact was moderate. Self-reporting of sensitivity by patients (66.7%) and dental examinations (66.3%) were the most frequently employed diagnostic tools. Dental caries (82.7%) and periodontal disease (74.5%) were most common conditions considered while performing differential diagnosis of DH. Education on adequate tooth brushing technique was regarded as the most common (74.1%) management strategy. Higher percentage of interns (68.2%) than students (31.8%) considered restorative treatment to manage DH (p=0.01). Results showed that 39.6% of respondents were either confident or very confident in treating the condition. DH is a frequently encountered oral condition in dental clinics. Participants demonstrated understanding about DH. However, majority lacked confidence in its successful management.
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Pandanus amaryllifolius (Roxb.) is a Southeast Asia tropical plant used in culinary and traditional medicine for century. It is a source of phytochemical constituents. The aim of the study was to evaluate the antimicrobial activity, antioxidant, and cytotoxic effect of the ethanol Pandanus amaryllifolius (Roxb.) leaf extracts for therapeutic potential in oral applications. To determine the amount of total phenolic contents and to evaluate the antioxidant activity of 70% ethanol leaf crude extracts of Pandanus amaryllifolius Roxb. the Folin-Ciocalteu method and the 2, 2-diphenyl-lpicrylhydrazyl (DPPH) assay were used. The ascorbic acid (vitamin C) and butylated hydroxytoluene (BHT) were used as standard positive controls. A broth serial micro dilution method was used to determine the minimum inhibitory concentration (MIC) against oral pathogens i.e. Porphyromonas gingivalis, Streptococcus mutans, Streptococcus sanguinis and Streptococcus salivarius compared to the standard antibiotics and 0.2% (w/v) chlorhexidine gluconate which were positive controls. The 3-(4,5-dimethylthiazolyl-2)-2,5- diphenyltetrazolium bromide reduction assay (MTT assay) was used for cytotoxicity effect tested. Results showed that the extracts contained 57.25 ± 0.02 mgGAE/g of total phenolic contents. The antioxidant activities of all tested substrates were calculated and exhibited as (IC50) the half maximum inhibitory concentration; is the concentration of antioxidant which reduces the free radical DPPH about 50%. The value of IC50 of ascorbic acid, the leaf extracts and BHT were 7.79 ± 1.25, 93.16 ± 10.46 and 110.57 ± 36.42 μg/mL,respectively. Pandanus amaryllifolius (Roxb.) leaf extracts had antimicrobial activities (MICs ranging from 32 to 125 μg/mL) μg/mL against tested oral bacteria. The extract exhibited the cytotoxicity effect on head and neck squamous cell carcinoma cell line (HNSCC cell line: HN31) with 50% lethal concentration (LC50) of 75.19 ± 3.48 μg/mL. Collective data from this study suggested the therapeutic potentials of the Pandanus amaryllifolius (Roxb.) for the future research and development health applications.
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Orthodontic treatment using fixed appliances is a predisposing factor for plaque accumulation because dental cleaning is more difficult. The lack of patient motivation and compliance in maintaining oral hygiene during treatment is also a predisposing factor in plaque build-up. Purpose: This study aimed to determine which motivation methods most influence Oral Hygiene Index scores based on the Plaque Index and the Gingival Index. The study is a randomized, single-blind clinical trial with concealed allocation. The subjects comprised patients treated with fixed orthodontic appliances. The patients' Oral Hygiene Index scores prior to and after being exposed to oral hygiene improvement motivation methods were evaluated. Statistical data processing was performed using univariate analysis, a Kruskal-Wallis test, a Wilcoxon test, and a one-way ANOVA. Three motivation methods were employed-modelling, a printed catalogue and a video. We found a significant difference between the modelling and video groups, followed by the catalogueand video groups. The video motivation method had the most significant effect on decreasing the Oral Hygiene Index score. The most effective method to increase the motivation of patients to maintain dental hygiene and improve plaque and gingival scores was the video method.
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p53 gene mutations observed at about 50 to 60 % in oral squamous cell carcinoma (OSCC). Until now, most of the studies were focused on exon 5 to 8 of p53 gene instead of the whole exons and it becomes a trend for p53 gene mutation study. However, the site of p53 gene mutation is still in controversy as available techniques showed different results. More importantly, the reports with the same technique demonstrated inconsistence mutation sites. The objective of the present study is to examine the status of p53 gene mutation in all exons (1 to 11). Recently, Multiplex Ligationdependent Probe Amplification (MLPA) technique has proven to be reliable and efficient for the detection of mutation. Therefore, present study used Multiplex Ligation-dependent Probe Amplification (MLPA) to examine p53 gene mutation from exon 1 to 11. DNA specimens from 58 OSCC patients and 10 healthy peoples (controls) were used in this study. Our results demonstrated that 31% (n=58/18) of OSCC patient have p53 gene mutation. Among them 56% (n=10/18) of mutation occurred in exon 3, followed by exon 4 which was 50% (n=9/18). Our study strongly indicated that exon 3 and 4 could be reliable and positive markers of OSCC development and progression. To our knowledge, this study for the first time showed that exon 3 is the hot spot region for p53 gene mutation in OSCC.
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The formation and development of the different dental markets and services, as well as numerous organizations with different forms of ownership, provoke the occurrence of quality control problem of various dental materials used in practice. Objective of research was to develop a method for the primary identification of changes in physical properties of photopolymer composite materials in comparison with features of reference samples, registered by the method of spectrophotometry. At the first stage of research official distributed composite syringes shades A1, A2 and A3 of different brands were collected. Prepared samples of investigated materials were placed inside spectrophotometer SF-2000, and indicators of absorption, transmission and reflection of light in the range of waves from 350 to 850 nm were recorded. At the second stage composite syringes of the same manufacturers and shades as in the first stage were collected, but without control of their official distribution. Subsequently, a comparison of the results of the study samples and reference database samples was performed in the adapted software. 96 experimental samples (83,48%) corresponded to the parameters previously registered in formed database of absorption, transmission and reflection in the range of 92,5-96,7% concordance (P＜0.05), 10 study samples (8,69%) corresponded to the reference values of 61,8-65,2% concordance (P＜0.05), 9 study samples (7,83%) corresponded to the reference values of 33,5-45,6% concordance (P ＜0.05). During analysis it was found that results of comparison transmission and reflective coefficients among reference and study samples were the closest at wavelength range of 550-600 nm and 800- 850 nm. Proposed approach helps to prevent the use of composite materials with non-sufficient physical properties registered by spectrophotometric method, samples of which could be presented at the grey market and objectively non attributed to any manufacture's brand.
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To assess the effect of filler on the presence of voids within resin-composite. Eight Light cure resin-composites were used in this study, seven models and one commercial composite. A disc (6mm in diameter and 2mm thickness) was used to prepare samples (n=8). Each sample was then scanned, reconstructed and analysed using Micro-CT. The percentages of voids obtained were then imported into SPSS and analysed using ANOVA and post hoc methods to check any significant differences between materials tested (p＜0.05). The Mean (SD) of % of voids for each group were obtained which ranged from 0.28% within Tetric Ceram to 3.48% within I4 model composite (1500nm). The different between means was statistically significant at p＜ 0.05. The filler size and distribution has an effect on the percentage of voids in resin composite which was greater with larger filler content.
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In recent years, there has been a noticeable increase in the awareness and interest in facial aesthetics. A pleasant profile is one of the main goals of orthodontic treatment. Orthodontic treatment with anterior retraction changes the soft tissues, particularly those relating to the lips and chin. In addition, soft tissue changes are also influenced by other factors, such as variations in the lip tonus, thickness, and length, sex, treatment mechanism, and racial ethnic background. Purpose: This study aimed to evaluate the soft tissue changes in the lips and chin after orthodontic treatment with anterior tooth retraction in the adult dolichofacial type. The subjects consisted of 34 patients, aged 17-35 years old, with the dolichofacial type (facial axis ＜ 90°), class I and class II malocclusion, and retraction of the anterior teeth (≥ 1 mm). The pre- and post-treatment cephalograms of the 34 patients were used to determine the pterygomaxillary line and for the Steiner analysis. The t-test, Pearson’s correlation, and multiple liner regression were used to analyze the pre- and post-treatment cephalograms. This study showed the differences in the soft tissue before and after orthodontic treatment with anterior tooth retraction in the dolichofacial type. Maxillary anterior tooth retraction had a significant effect on the upper lip thickness, upper lip position, and lower lip position changes, while mandibular anterior tooth retraction had a significant effect on the lower lip thickness changes. Anterior tooth retraction had a strong correlation with the changes in the lip thickness and lip positions in the dolichofacial type patients.
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Dental trauma relates to negative consequences that the child links to negative experience. The aim of this study was to assess the scale of dental anxiety in children with dental trauma and to evaluate the questionnaires as measurement scales which handle the trauma and anxiety parameters. Study included 505 patients, aged 7 to 14 years of whom 254 children experienced dental trauma and 251 children were without dental trauma experience. The subjects came for dental appointment from 2015 to 2016 and filled out the questionnaires about anxiety. The following questionnaires were used: CFSS-DS, CDAS, CMFQ, S-DAI and DVSS-SV. The Cronbach alpha was used to check the reliability and validity of the questionnaires. Larger percentage of cases had lower scale of dental anxiety. Meanwhile high scale dental anxiety was proportionally higher in the group with dental trauma for all questionnaires. Dental visit satisfaction scale was higher in the group without dental trauma. Female patients showed higher scores for all tests. In the group of patients with dental trauma, the mean values of anxiety scale were also higher in females. Also, dental visit satisfaction scale was higher in females. According to the age of children the only significant correlation between the patient's age and dental anxiety was ascertained in S-DAI test in the group without dental trauma (r=-0.137, p=0.03). All questionnaires were evaluated as reliable and valid for anxiety determination. In children with dental trauma the highest Cronbach alpha coefficient was found for S-DAI (0.991) and CFSS-DS (0.974). In children without dental trauma the highest Cronbach alpha coefficient was found for S-DAI (0.966), CFSSDS (0.959) and CDAS (0.914). The mean anxiety score was higher in children with dental trauma, as well as in the girls and children who are more afraid of medical interventions (CMFQ). All tests showed good reliability of estimating anxiety and could be used in every day practice.