Larval Migratory Behavior of Long-term-maintained Toxocara canis Embryonated Eggs in Mice

長期培養之犬蛔蟲受孕卵內幼蟲於小鼠的移行行為

范家堃(Chia-Kwung Fan) ； 林永和(Yun-Ho Lin) ； 洪健清(Chien-Ching Hung) ； 蘇霩靄(Kua-Eyre Su)

《臺灣獸醫學雜誌》 30卷2期 (2004/06) Pp. 99-105

https://doi.org/10.7009/TVJ.200406.0099

摘要

藉幼蟲回收研究評估儲存於2%福馬林液內，於4℃下保存14個月之犬蛔蟲受孕卵感染小鼠第一天至第四百六十九天後，幼蟲於小鼠體內的移行行為。每次犧牲三隻感染鼠與二隻同齡未感染鼠，對肝臟和肺臟（肝肺期）與肌肉和腦部（神經肌肉期）組纖以酸性的胃蛋白酵素進行消化。自肝肺期的幼蟲回收於感染後第五天達到最高，而非感染後的第二天，此後，大部分的幼蟲轉為移行至神經肌肉期。就統計學而言，肝肺期與神經肌肉期的幼蟲回收與時間分別成負與正相關（r = - 0.688，P = 0.005；r = 0.138，P = 0.327）。綜合而言，儘管14個月培養的犬蛔蟲幼蟲似乎延遲移行至肝肺期，大部分仍出現於神經肌肉期，特別是腦部。

關鍵字

犬蛔蟲 ； 幼蟲移行行為 ； 小鼠

並列摘要

Larval migratory behavior of Toxocara canis embryonated eggs that had been maintained in 2% formalin for 14 months at 4℃ was evaluated using a larval recovery study in mice at between 1 and 469 days post infection (DPI) . Three infected mice and 2 aged-matched uninfected control mice were sacrificed daily for acid-pepsin digestion of the liver and lungs (hepatopulmonary phase) as well as the musculature and brain (myotropic-neurotropic phase). Larval recovery from the hepatopulmonary phase reached a peak at 5 DPI, not at 2 DPI; thereafter, they began to migrate to the myotropic-neurotropic phase. Statistically, larval recoveries from the hepatopulmonary and myotropic-neurotropic phases respectively showed negative and positive correlations with time (r = - 0.688, P = 0.005; r = 0. 138, P = 0.327). Altogether, although there seemed to be a delay in the migration of 14-month-cultured T. canis larvae to the hepatopulmonary phase, most of them still exhibited the myotropic-neurotropic phase, especially in the brain.

並列關鍵字

Larval migratory behavior ； Mice ； Toxocara canis