- 期刊
芒果種原親緣關係之研究
Study on Genetic Variations of Mango (Mangifera indica L.) Germplasm
摘要
本文以逢機擴大多型性DNA(RAPD)進行芒果種原間的遺傳相關性分析，利用200條逢機引子對鳳山分所收集之40個芒果品種（系）進行分析，結果顯示在這些引子中有22個引子所產生之條帶具有品種間多型性，且有高重複性。所有參試品種（系）無法僅利用一個引子來區分，需要組合數個引子才可區分個別品種（系）。綜合各引子所產生條帶之叢群分析結果顯示，40個供試品種（系）可以分為3群，各群相似度介於53.3至88.1%之間，平均值為70.3%。其中親緣最相似的是E10-1和玉文6號，其相似度為88.1%。差異最大的為香檨和Vanary品種之相似度僅53.3%，而Dasheri和香檨2品種與其他群之相似度低，顯示此2品種與其他品種之親緣關係較遠。
並列摘要
Genetic relationships among cultivars (or lines) of mango (Mangifera indica L.) were analyzed using RAPD markers. Forty mango cultivars (or lines) that planted at the Fengshan Tropical Horticultural Experimental Branch, Taiwan Agricultural Research Institute, Fengshan, Kaohsiung, were examined using 200 random primers. Results showed that 22 of the 200 primers were inter-species polymorphism with high consistency. A combination of several primers was required for identification of cultivars (or lines). Results of RAPD cluster analyses showed that the 40 mango cultivars (or lines) were divided into 3 groups and the genetic similarity among these groups ranged from 53.3 to 88.1%, averaging 70.3%. The E10-1 and Yu-win No.6 had the highest degree of similarity (88.1%), whereas the Pung-Swain and Vanary had low degree of similarity (53.3%). However, Dasheri and Pung-Swain were highly different from all others, suggesting that they are highly diversed genotypes.
延伸閱讀
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