簡訊：豬傳染性胃腸炎（TGE）在台灣再度爆發

自1996年初開始，台灣許多豬場都報告其在養豬群有急性水樣下痢，並造成出生仔豬大量死亡的疫情。本研究病材來自從南到北8個豬場，剖檢發病仔豬，發現腸道變薄、充滿水樣液體且胃有凝乳。結腸內容以電子顯微鏡負染色檢查，發現有大小約100-160 nm的冠狀病毒及一些30-40 nm的腸病毒顆粒。利用抗TGEV核酸蛋白的單株抗體做冷凍切片間接螢光染色，或／及石蠟包埋切片做ABC組織免疫染色，在萎縮的絨毛上皮細胞質內可觀察到明顯螢光或黑褐色素沉著的陽性反應，証明主要病原為TGEV。TGEV的突然爆發其原因推測可能有：一是自然的週期性流行，二是來自境外購入之種豬帶有病毒，三是原來本土TGEV產生變異。造成此次發病的病毒株，其表面蛋白的胺基端以及ORF 3a/3b區域與以往的分離株是否有抗原性以及基因的變異，則仍有待進一步的探討以推究此次爆發的真正原因。

關鍵字

豬傳染性胃腸炎

並列摘要

Many pig farms, despite their geographical location in Taiwan, reported to suffer from severe infectious diarrhea since January 1996. The owner complained that after the first sign of watery diarrhea observed in the finishing unit or farrowing unit, it spread to farrowing house within few days and caused tremendous loss as a result of the death mainly in the piglets less than 7 days of age. The necropsy from the infected piglets revealed milk clot in the stomach and small intestine was becoming very thin and filled with watery content. Coronavirus or coronavirus-like virus particles as well as some 30-40 nm enterovirus were observed by EM from the negative stained colon content. Indirect fluorescent stain of frozen tissue sections and ABC stain of formaline-fixed paraffin embedded tissue sections by using anti-TGEV nucleoprotein monoclonal antibody showed positive fluorescent or brownish black coloration in the epithelial cells of the atrophic villi, it was believed the major etiological agent was TGEV. Three possibilities were proposed for the emerge of TGEV in Taiwan from this outbreak; first, a normal epidemiological cycle of TGE; second, an imported breeding stock purchased abroad; third, a new virulent TGEV strain was emerged. Any antigenic or sequence differences within the N-terminal of the TGEV S protein and orf 3a/3b region of the newly isolated TGEV strain from this outbreak will be compared with our previous isolate TGEV strain to verify the potential new TGEV etiology.

並列關鍵字

TGE ； Transmissible gastroenteritis

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簡訊：豬傳染性胃腸炎（TGE）在台灣再度爆發

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