- 期刊
Eicosanoid Production and Cytokine mRNA Levels in Piglets Fed with Various Edible Oils
不同油脂對仔豬細胞素基因表現與發炎介質生成之影響
摘要
To investigate the interactions between cytokine gene expressions and eicosanoid synthesis, 16 piglets were randomized into four groups and fed diets containing 3.6% coconut oil, fish oil, lard, or soybean oil plus 0.4% soybean oil for 21 days to modulate the synthesis of eicosanoids including leukotriene (LT), prostacyclin (PG), and thromboxane (TX) in the lungs. Interleukin-4 (IL-4), interleukin-8 (IL-8), and interferony-γ (IFN-γ) gene expressions in spleen and thymus were examined using RT-PCR. The results showed that fish oil effectively inhibited the synthesis of PG and TX (P＜0.05) in lungs but did not reduce the synthesis of pulmonary LT. Interleukin-4, IL-8, and IFN-γ gene expressions in the spleen and thymus were not affected by dietary fat. However, a significant positive correlation was observed between LT synthesis in the lung and IL-4 gene expression in the spleen (r=0.80, P=0.0006). Stimulated-LT production in lungs tended to be positively correlated to splenic IL-8 gene expression (r=0.60, P=0.057). Negative correlation was found between IL-4 and IFN-γ gene expressions in the spleen. The mRNA levels of IFN-γ were also negatively correlated to those of IL-8 in the spleen. Therefore, the positive correlation between splenic IL-4 gene expression and the release of LT in the lungs was consistent with clinical features in asthmatic patients. In contrast, the negative correlation between IL-4 and IFN-γ gene expressions in spleen idicated that IFN-γ and IL-4 gene expressions in the spleen were apparently converse.
並列摘要
To investigate the interactions between cytokine gene expressions and eicosanoid synthesis, 16 piglets were randomized into four groups and fed diets containing 3.6% coconut oil, fish oil, lard, or soybean oil plus 0.4% soybean oil for 21 days to modulate the synthesis of eicosanoids including leukotriene (LT), prostacyclin (PG), and thromboxane (TX) in the lungs. Interleukin-4 (IL-4), interleukin-8 (IL-8), and interferony-γ (IFN-γ) gene expressions in spleen and thymus were examined using RT-PCR. The results showed that fish oil effectively inhibited the synthesis of PG and TX (P＜0.05) in lungs but did not reduce the synthesis of pulmonary LT. Interleukin-4, IL-8, and IFN-γ gene expressions in the spleen and thymus were not affected by dietary fat. However, a significant positive correlation was observed between LT synthesis in the lung and IL-4 gene expression in the spleen (r=0.80, P=0.0006). Stimulated-LT production in lungs tended to be positively correlated to splenic IL-8 gene expression (r=0.60, P=0.057). Negative correlation was found between IL-4 and IFN-γ gene expressions in the spleen. The mRNA levels of IFN-γ were also negatively correlated to those of IL-8 in the spleen. Therefore, the positive correlation between splenic IL-4 gene expression and the release of LT in the lungs was consistent with clinical features in asthmatic patients. In contrast, the negative correlation between IL-4 and IFN-γ gene expressions in spleen idicated that IFN-γ and IL-4 gene expressions in the spleen were apparently converse.
延伸閱讀
- Liu, B. H. (2009). 豬脂締素及其受體表現調控機制與功能之探討 [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2009.00789
- 劉芳爵、姜延年、沈添富（2005）。蛋白質來源對離乳仔豬胃與胰脂肪酶活性發展之影響。畜產研究，38(2)，153-163。https://www.airitilibrary.com/Article/Detail?DocID=P20200930001-200506-202011160010-202011160010-153-163
- 許博淳（2014）。豬隻不同品種及部位肌肉之脂質代謝相關基因表現〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2014.01071
- 蘇天明、劉建甫、邱啟明、蔡金生、廖宗文、盧金鎮（2006）。不同品種與屠宰體重對豬隻背最長肌化學組成分、肉色及脂肪酸組成之影響。畜產研究，39(2)，111-119。https://www.airitilibrary.com/Article/Detail?DocID=P20200930001-200606-202011130010-202011130010-111-119
- 洪嘉良（2018）。不同副豬嗜血桿菌母源移行抗體對仔豬之影響〔碩士論文，國立屏東科技大學〕。華藝線上圖書館。https://doi.org/10.6346/THE.NPUST.VM.005.2018.D07