應用農桿菌進行台中亞蔬四號番茄之轉殖

本試驗係以子葉或下胚軸爲培植體，建立臺灣栽培種番茄台中亞蔬四號品種之農桿菌媒介法轉殖系統。番茄之子葉或下胚軸與含質體pBI121的農桿菌LBA 4404共培養後，於含有100mg/l kanamycin的培養基上進行篩選及培養，可獲得擬轉殖枝條。部分具有kanamycin抗性的技條，可表現GUS活性。擬轉殖枝條培養於含有100mg/l kanamycin之發根培養基，待發根形成植株後，移至黑軟盆中種植。藉由GUS活性分析、聚合酶連鎖反應與南方氏核酸雜交分析，證實外來基因已穩定轉殖於番茄植株中並可表現。

關鍵字

GUS基因；聚合酶連鎖反應；南方氏核酸雜交分析

並列摘要

A transformation system using cotyledon and hypocotyl segment as explant was developed for the tomato variety ”Taichung Asveg No.4”. Cotyledon and hypocotyl segments of tomato were co-cultivated with Agrobacterium tumefaciens LBA 4404 harboring the binary plasmid pBI 121 (conferring β-glucuronidase activity and resistance to kanamycin) and cultured on the medium supplemented with 100 mg/l kanamycin to form shoots and select putatively transformants. The plants showing kanamycin-resistant and GUS (β-glucuronidase) activity were rooted on the medium containing 100 mg/l kanamycin, and transferred to puts. Stable integration and expression of the transgenes were confirmed by GUS assay, polymerase chain reaction (PCR), and Southern hybridization analysis.

並列關鍵字

GUS gene ； polymerase chain reaction ； Southern analysis

延伸閱讀

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王啟正、林立、林學詩（2014）。番茄‘花蓮亞蔬五號’以農桿菌法轉殖＂Bt＂基因之研究。花蓮區農業改良場研究彙報，(32)，15-27。https://www.airitilibrary.com/Article/Detail?DocID=10184457-201412-201501230026-201501230026-15-27
林天枝、洪澨堂（2001）。番茄台中亞蔬十號新品種之育成。臺中區農業改良場特刊，()，26-26。https://doi.org/10.29563/ZHWHGX.200112.0025
Ann, P. J., Tsai, J. N., Wang, T. C., Chen, C. H., Lin, M. J., & Ko, W. H. (2010). Reevaluation of the Report of the A2 Mating Type of Phytophthora infestans on Tomato in Taiwan. Botanical Studies, 51(2), 203-207. https://www.airitilibrary.com/Article/Detail?DocID=1817406X-201004-201006070071-201006070071-203-207

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應用農桿菌進行台中亞蔬四號番茄之轉殖

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