菜鴨濾泡類固醇生成與酵素及相關蛋白之表現

Expression of Steroidogenic Enzymes and Related Protein in Follicles of Tsaiya Duck

邱智賢(Chih-Hsien Chiu) ； 林政緯(Cheng-Wei Lin) ； 鍾德憲(De-Shien Jong) ； 郭文旺(Wen-Wang Kuo) ； 林依亭(I-Ting Lin) ； 吳兩新(Leang-Shin Wu)

《中華農學會報》 8卷5期 (2007/10) Pp. 456-469

https://doi.org/10.6730/JAAC.200710_8(5).0005

摘要

The purpose of this study was to investigate the correlation of steroidogenesis with the expression of steroidogenic enzymes in preovulatory follicles of Tsaiya duck (Ana platyrhynchos var. domestica), Preovulatory follicles (F1-F5) were collected and the granulosa and theca layers o follicles were isolated. The concentration of progesterone (P4), testosterone (T) and estradiol (E2) were detected with EIA method. The expression of StAR (steroidogenic acute regulatory) protein. P450scc (P450 cholesterol side-chain cleavage enzyme), 3β-HSD (3β-hydroxysteroid dehydrogenase) and P450arorn (P450 aromatase) were measured. The results showed that progesterone content in the granulosa layer was higher than in the theca layers, especially in F1 and it was increased gradually with the maturity of the follicle. In contrast, the T and E2 contents of theca layers were higher than granulosa layers. The T and E2 contents of the F4/5 layer were the highest in different stages (P＜0.05), but there was no difference in granulosa layers obtained from different stages of preovulatory follicles. The expression of StAR, P450scc and 3β-HSD was the highest in F1 granulosa layer and the expression levels gradually increased with the maturity of follicles. However, the expression level of P450arom showed no significant difference in different stages of granulosa layer development. In theca layers of preovulatory follicles, expression of StAR, P450scc and 3β-HSD showed no difference, hut the P450arom expression of F4/F5 was significantly higher than in other stages. In brief, it could be concluded that: (1) the biosynthesis of P4 is positively correlated with the expression of key steroidogenic enzymes (StAR, P450scc and 3β-HSD) in preovulatory follicles of Tsaiya duck; (2) the F1 granulosa layer mainly secretes P4 by increased enzymatic activity and expression of StAR, P450scc and 3β-HSD that induce ovulation; (3) the F3 and F4/F5 of theca layers produce a high level of T, but the expression level of StAR was not different.

關鍵字

濾泡 ； 類固醇生成 ； 類固醇生成酵素 ； 菜鴨

並列摘要

The purpose of this study was to investigate the correlation of steroidogenesis with the expression of steroidogenic enzymes in preovulatory follicles of Tsaiya duck (Ana platyrhynchos var. domestica), Preovulatory follicles (F1-F5) were collected and the granulosa and theca layers o follicles were isolated. The concentration of progesterone (P4), testosterone (T) and estradiol (E2) were detected with EIA method. The expression of StAR (steroidogenic acute regulatory) protein. P450scc (P450 cholesterol side-chain cleavage enzyme), 3β-HSD (3β-hydroxysteroid dehydrogenase) and P450arorn (P450 aromatase) were measured. The results showed that progesterone content in the granulosa layer was higher than in the theca layers, especially in F1 and it was increased gradually with the maturity of the follicle. In contrast, the T and E2 contents of theca layers were higher than granulosa layers. The T and E2 contents of the F4/5 layer were the highest in different stages (P＜0.05), but there was no difference in granulosa layers obtained from different stages of preovulatory follicles. The expression of StAR, P450scc and 3β-HSD was the highest in F1 granulosa layer and the expression levels gradually increased with the maturity of follicles. However, the expression level of P450arom showed no significant difference in different stages of granulosa layer development. In theca layers of preovulatory follicles, expression of StAR, P450scc and 3β-HSD showed no difference, hut the P450arom expression of F4/F5 was significantly higher than in other stages. In brief, it could be concluded that: (1) the biosynthesis of P4 is positively correlated with the expression of key steroidogenic enzymes (StAR, P450scc and 3β-HSD) in preovulatory follicles of Tsaiya duck; (2) the F1 granulosa layer mainly secretes P4 by increased enzymatic activity and expression of StAR, P450scc and 3β-HSD that induce ovulation; (3) the F3 and F4/F5 of theca layers produce a high level of T, but the expression level of StAR was not different.

並列關鍵字

Follicle ； Steroidogenesis ； Steroidogenic enzymes ； Tsaiya duck