山羊於動情周期間黃體孕酮生成能力之探討

The Study of Progesterone Secretary Profile of Corpus Luteum during the Estrous Cycle in Goat

邱智賢(Chih-Hsien Chiu) ； 曾泰祥(Tai-Hsiang Tseng) ； 莊叡豐(Ruei-Feng Chuang) ； 陸玉玲(Yu-Ling Lu) ； 吳兩新(Leang-Shin Wu)

《台灣農學會報》 10卷4期 (2009/08) Pp. 321-334

https://doi.org/10.6730/JAAT.200908_10(4).0005

摘要

黃體是雌性哺乳動物於動情周期與懷孕時的暫時性組織。當卵母細胞及其周圍卵丘細胞自成熟的濾泡中釋出，意即排卵後，構成一新的功能單位即為黃體。本試驗利用山羊黃體組織為材料，以離體(in vitro)之細胞為試驗模式，利用體外建立之初代細胞培養系統，檢測動情周期間山羊黃體細胞類固醇生成能力，並探討不同動情周期間採集之黃體細胞受激後之孕酮分泌能力變化。因此，對於已進入細胞凋亡時期的黃體細胞，是否依然具有類固醇生成能力是本試驗探討的重點。試驗結果顯示，隨著動情周期的進行，黃體體積與重量達到發育尖峰後開始退化而解體，以及孕酮(progesterone, P4)分泌亦急速下降等，其間黃體重量及孕酮分泌的下降是黃體解體時的重要指標。在山羊動情周期間，卵巢與黃體重量變化與黃體細胞孕酮分泌能力成正比，而中期之黃體細胞與後期之黃體細胞其RNA合成能力相差甚大，但與蛋白質合成能力比較後，則顯示其差異主要為功能性蛋白表現的差異。此外，後期黃體細胞，對於刺激胞內PKC路徑試劑反應明顯上升。

關鍵字

黃體 ； 類固醇生成 ； 山羊

並列摘要

The corpus luteum (CL) is short-living, one of the temporary endocrine glands of female mammals, which develops from the postovulatory follicles after ovulation. It is known as the foremost gland for the synthesis of progesterone (P4), which plays a vital role in the establishment and maintenance of pregnancy. After ovulation, dramatic structural changes like the formation of numerous blood vessels (angiogenesis) and rapid transition of follicle cells which form into new functional units are called corpus luteum. We established a new in vitro experimental model to study the regulatory mechanisms of progesterone biosynthesis in caprine corpus luteum. The main purpose of this study was to examine the changes of steroidogenic capacity in caprine corpora lutea throughout the estrous cycle by using in vitro strategy. The results showed that the high correlation between the difference in ovarian weight and quantitative changes in luteal weights was directionally proportional to the progesterone secreting ability of luteal cells. In fact, the expression of RNA levels of middle and latter stages of luteal cells were apparently different. But surprisingly, the comparative study of the protein synthetic ability showed significant differences in the functional protein expressions in the latter luteal cells. In addition, the dramatic decreases of progesterone levels in the later stage of corpus luteum may be due to the high sensitivity of PKC-mediated steroidogenic pathways.

並列關鍵字

Corpus luteum ； Steroidogenesis ； Goat