實驗用囓齒動物飼料配方AIN-93G之鐵質生體可用率

Iron Bioavailability of AIN-93G Purified Diets for Laboratory Rodents

張美鈴(Mei-Ling Chang) ； 彭珍芳(Jen-Fang Perng) ； 蕭寧馨(Ning-Sing Shaw)

《中華民國營養學會雜誌》 23卷3期 (1998/08) Pp. 239-250

https://doi.org/10.6691/JCNS.199808_23(3).0003

摘要

The American Institute of Nutrition published the AIN-76 purified diets for laboratory rodents in 1977. As the diet was used over the years and some problems were discovered, the AIN-93 diets were formulated as substitutes. Some major differences in the formulation of AIN-93G compared with AIN-76 are the reduction of the amount of phosphorous from 5g/kg diet to 3g/kg diet, replacement of CaHPO4 with CaCO3 and KH2PO4, and increased Ca:P molar ratio from 0.75 to 1.3. Since calcium salts and Ca:P molar ratio may affect iron absorption, our objective was to compare the iron bioavailability of AIN-76 and AIN-93G diets using a rat hemoglobin repletion bioassay. Weanling male Wistar rats were first rendered anemic and fed AIN-76 diets or AIN-93G diets supplemented with 6, 12, 18ppm iron supplied as ferrous sulfate for 14 days. The major effect of diet types on repleted hemoglobin concentrations and the gain of hemoglobin iron was not significant (P＞0.1) based on two-way ANOVA. Significant linear dose-response correlation was obtained between hemoglobin gains and added iron concentrations, and between hemoglobin iron gains and intake of added iron by regression analysis at P=0.0001. Calculated from the regression coefficient of the latter, the relative biological value of iron for AIN-93G diets was 94.5. It is concluded that iron bioavailability of AIN-93G is similar to that of AIN-76.

關鍵字

鐵生體可用率 ； 囓齒動物 ； AIN-76 ； AIN-93G ； 血紅素再生法

並列摘要

The American Institute of Nutrition published the AIN-76 purified diets for laboratory rodents in 1977. As the diet was used over the years and some problems were discovered, the AIN-93 diets were formulated as substitutes. Some major differences in the formulation of AIN-93G compared with AIN-76 are the reduction of the amount of phosphorous from 5g/kg diet to 3g/kg diet, replacement of CaHPO4 with CaCO3 and KH2PO4, and increased Ca:P molar ratio from 0.75 to 1.3. Since calcium salts and Ca:P molar ratio may affect iron absorption, our objective was to compare the iron bioavailability of AIN-76 and AIN-93G diets using a rat hemoglobin repletion bioassay. Weanling male Wistar rats were first rendered anemic and fed AIN-76 diets or AIN-93G diets supplemented with 6, 12, 18ppm iron supplied as ferrous sulfate for 14 days. The major effect of diet types on repleted hemoglobin concentrations and the gain of hemoglobin iron was not significant (P＞0.1) based on two-way ANOVA. Significant linear dose-response correlation was obtained between hemoglobin gains and added iron concentrations, and between hemoglobin iron gains and intake of added iron by regression analysis at P=0.0001. Calculated from the regression coefficient of the latter, the relative biological value of iron for AIN-93G diets was 94.5. It is concluded that iron bioavailability of AIN-93G is similar to that of AIN-76.

並列關鍵字

iron bicavailability ； rodents ； AIN-76 ； AIN-93G ； hemoglobin repletion bioassay