THE Surgical Destabilization of the Medial Meniscus (DMM) Model of Osteoarthritis in the BALA/C Mouse: MMP-9 as a Prognostic Maker: A Pilot Study

Purpose: Osteoarthritis (OA), a chronic degenerative joint disorder characterized by articular cartilage destruction and osteophyte formation, is a major cause of disability. The severity and location of lesions following surgical destabilization of the medial meniscus (DMM) model are consistent with those observed in aged spontaneous mouse models of OA. Further, the DMM model has adequate sensitivity to indicate disease modification. This study was designed to evaluate the possibility of formulating a therapeutic reference paradigm for matrix metalloproteinase (MMP)-9 in the DMM model. Methods: BALB/C mice, 6-8 weeks old, received surgical DMM, and were randomly divided into three groups (normal, sham, and DMM). The mice were sacrificed 2 months later. The synovium of the knee joint was harvested for real-time polymerase chain reaction amplification. Pathological examination of the knee joints was then performed. Results: There was a significant difference in the Mankin score of the knee joint as well as in the relative expression level of MMP-9 in the synovium of the knee joint between the 3 groups (P=0.0246 (sham between DM); P＜0.001 (normal group between DM group).There was a significant correlation between the Mankin score and the relative expression levels of MMP-9 in the knee joints (DMM (r=0.9486, P=0.0256); sham (r=0.9176, P=0.014)). Conclusions: The results of this study imply that the relative expression level of MMP-9 in the synovium of knee joint might be a useful prognostic marker for DMM.

並列關鍵字

matrix metalloproteinase-9 ； synovium ； osteoarthritis ； DMM ； marker ； clinical

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