對流式低溫顯微鏡冷凍台與溫控系統之設計

Design of the Convection Cryomicroscope Cold Stage and Temperature Controller

王建勝(Cheng-Sheng Wang) ； 林達德(Ta-Te Lin)

《農業機械學刊》 1卷1期 (1992/01) Pp. 42-49

https://doi.org/10.30062/JAM.199201.0005

摘要

低溫顯微鏡系統主要應用於直接觀察生物細胞在低溫下之形態變化及凍結特性，以促進低溫生物學之基礎研究與細胞低溫冷凍技術之發展。本研究之目的在研製低溫顯微鏡系統之對流式冷凍台及製作其微電腦介面溫控系統。溫度控制系統以熱電偶量取冷凍台溫度，訊號經由本研究所設計之AD/DA介面讀取送入微電腦後以PID法則控制相位加熱電路，以調整冷凍台溫度。溫控系統在4瓦特功率下，最大加熱速率可以到達100℃/min，最大的冷卻速率為80℃/min，恆溫控制的最大正負誤差為1.2℃。對流式冷凍台與溫控系統製作完成後，實際進行升溫與降溫之溫控實驗以測試系統之性能，最後並以甘薯原生質體為對象進行低溫實驗，成功地觀察到其冷凍與解凍過程中之形態變化。

關鍵字

低溫顯微鏡 ； 冷凍台 ； 溫度控制 ； 原生質體

並列摘要

The cryomicroscope system is usually used to observe the low temperature behavior of biolgical cells during freezing and thawing. The purpose of this research is to design a convection cold stage and a micro-computer based temperature controller for the cryomicroscope system. A foil thermal couple and a thermal resistor were use to measure the temperature of the cold stage. The generated voltage signal was then fed to a microcomputer using an AD/DA interface developed in this research. PID algorithm and a phase-heating circuit were applied for the temperature control. The maximum heating rate and cooling rate of the cold stage are 100℃/min and -80℃/min, respectively. The maximum error of constant temperature control were 1.2℃. Experiments were performed to test the performance of the cold stage and the temperature control system. Freeze-thaw cycle of sweet potato protoplasts were observed in real time using the developed system.

並列關鍵字

Cryomicroscope ； Cold Stage ； Temperature Control ； Protoplasts