微陣列晶片於核酸適體研究之應用

Applications of Microarray in Aptamer Study

勞業興(Yeh-Hsing Lao) ； 陳林祈(Lin-Chi Chen) ； 張翼中(Yi-Chung Chang) ； 紀鈞瑋(Chun-Wei Chi) ； 白果能(Ko-Nan Peck)

《科儀新知》 172期 (2009/10) Pp. 95-102

https://doi.org/10.29662/IT.200910.0013

摘要

“適體”泛指具有抗體功能之單股寡核酸，其可形成特殊之立體結構以辨識特定標的蛋白質，深具疾病診斷與治療之應用潛力。適體的產生藉由一種稱為SELEX法的人工篩選程序，此程序涉及大量核酸序列分析與配位子確認，因此應用微陣列晶片技術可有效地縮短核酸適體篩選時程。另一方面，以寡核酸所構成之適體點印成微陣列晶片可作為一種新穎蛋白質晶片應用。綜合上述原由，本文將簡介適體研發背景，並藉由幾個適體研究上的應用實例，探討微陣列晶片技術在加速適體篩選與蛋白質分析檢測方面之優勢。

並列摘要

”Aptamers” are a unique class of single-stranded oligonucleotides that resemble naturally occurring antibodies. They can fold into unique tertiary conformations for specific recognition of target proteins. Thus, aptamers have great potential for disease diagnosis and therapy. Generation of an aptamer relies on an in vitro selection process called SELEX, which involves analysis and identification of ligands from a multitude of nucleic acid sequences. Hence, exploitation of microarray techniques can effectively shorten the time required for aptamer screening. In addition, arraying oligonucleotide-based aptamers into a microarray format can lead to a novel protein chip application. Considering the aforementioned reasons, we brief the backgrounds for aptamer R&Ds and discuss the strengths of microarray techniques in acceleration of aptamer screening and protein analysis by several practical examples in this article.