台灣地區竹園釘線蟲Gracilacus bilineate和新記錄種G. aculenta (Nematoda: Criconematoidea, Tylenchulidae)之鑑定

Indentification of Gracilacus bilineata and G. aculenta (Nematoda: Criconematioidea, Tylenchulidae) among Bamboo Plantations in Taiwan

陳殿義(D. Y. Chen) ； 倪蕙芳(H. F. Ni) ； 蔡東纂(T. T. Tsay) ； 顏志恒(J. H. Yen)

《植物病理學會刊》 17卷3期 (2008/09) Pp. 209-220

https://doi.org/10.6649/PPB.200809_17(3).0004

摘要

自2005年9月起，從台灣地區7處竹園(Bambusa spp.)的根圈土壤中，以改良式柏門氏漏斗分離法，總計分離到9群釘線蟲(Gracilacus spp.)。依據外觀形態特徵、形態測量值(morphometrics)及包括完整5.8S基因、內轉錄區間ITS-1和ITS-2及部分18S和28S基因的核糖體DNA(ribosomal DNA, rDNA)片段序列相似度比對分析後，其中5群鑑定爲G. bilineata Brzeski，1995，另4群鑑定爲G. aculenta(Brown, 1959)Raski，1962，爲台灣地區的新記錄種。本研究以SEM可清楚地觀察到G. bilineata和G. aculenta的側帶上具有3條側線和小型的陰門蓋構造，並首次在GenBank中登錄此二種釘線蟲的rDNA片段代表性序列，其序列大小依序分別爲1036 bp和1021 bp，而二者間的序列相似度爲86.0%。

關鍵字

釘線蟲 ； 竹 ； 鑑定 ； 陰門蓋 ； 核糖體DNA ； Gracilacus bilineata ； Gracilacus aculenta

並列摘要

Nine populations of Gracilacus spp. have been isolated by modified Baermann funnel method from the rhizosphere soil of bamboo plants among 7 plantations its Taiwan since September, 2005. Comparisons of the morphology, morphometrics and ribosomal DNA fragment sequences (include full 5.8S gene, ITS-1, ITS-2 and partial 18S and 28S gene) have yielded 5 populations as G. bilineata Brizeski, 1995 and 4 populations as G. as aculenta (Brown, 1959) Raski, 1962. G. aculenta was the first report in Taiwan. In this study, additional SEM photographs clearly revealed 3 incisures on lateral field, and the existence of small vulval flaps for these two pin nematode species. The representative sequences of rDNA fragment of G. bilineata and G. aculenta had the size of 1036 hp and 1021 hp, respectively, and were firstly submitted to GenBank.

並列關鍵字

pin nematode ； bamboo ； identification ； vulval flaps ； ribosomal DNA ； Gracilacus bilineata ； Gracilacus aculenta