3-Aminobenzamide對過氧化氫誘發大鼠支氣管肺泡沖洗細胞之DNA單股斷裂之修復及細胞死亡形式之影響

Effects of 3-Aminobenzamide on the Repairing of DNA Single Strand Breaks and the Modes of Cell Death in Rat Bronchoalveloar Lavage Cells Exposed to Hydrogen Peroxide

陳永俐(Yung-Li Chen) ； 鄭穹翔(Chiang-Hsiang Chung) ； 李建穀(Jiann-Gwu Lee)

《臺灣獸醫學雜誌》 33卷2期 (2007/06) Pp. 114-122

https://doi.org/10.7009/TVJ.200706.0114

摘要

This study was focused on the influences of 3-Aminobenzamide (3-AB), an inhibitor to poly (ADP-ribose) polymerase (PARP), on the DNA single strand break and repair, and the modes of cell death in rat Bronchoalveloar lavage cells (RBALC) following hydrogen peroxide (H2O2) exposure. By single cell gel electrophoresis assay, RBALC DNA single strand breaks were significantly increased as H2O2 concentration raised (p＜0.05). Repairing of DNA single strand breaks were both significantly inhibited after addition of 3-AB for either 3 or 6 hours to RBALC that were previously exposed to H2O3 (p＜0.05). Trypan blue excluding tests revealed significant increases in the survival rates of H2O2-treated RBALC after co-incubated with 3-AB for both 3 and 6 hours (p＜0.05). Addition of 3-AB was also capable of shortening the onset of DNA laddering pattern (indication of apoptosis at early stage) by 6 hours and DNA laddering pattern peaked after 9 hours co-incubation with 3-AB. Morphologically, 3-AB did not switch cell death from necrosis to apoptosis; however, up to 30% of decrement in survived RBALC population could be observed after 12 hours repairing with 3-AB. These results suggested that 3-AB inhibited repairing of DNA single strand breaks induced by H2O2, it also might postpone cellular necrosis and redirected the cells to apoptosis by inhibiting PARP activities.

關鍵字

PARP ； 3-Aminobenzamide ； DNA單股斷裂 ； 細胞凋亡 ； 壞死

並列摘要

This study was focused on the influences of 3-Aminobenzamide (3-AB), an inhibitor to poly (ADP-ribose) polymerase (PARP), on the DNA single strand break and repair, and the modes of cell death in rat Bronchoalveloar lavage cells (RBALC) following hydrogen peroxide (H2O2) exposure. By single cell gel electrophoresis assay, RBALC DNA single strand breaks were significantly increased as H2O2 concentration raised (p＜0.05). Repairing of DNA single strand breaks were both significantly inhibited after addition of 3-AB for either 3 or 6 hours to RBALC that were previously exposed to H2O3 (p＜0.05). Trypan blue excluding tests revealed significant increases in the survival rates of H2O2-treated RBALC after co-incubated with 3-AB for both 3 and 6 hours (p＜0.05). Addition of 3-AB was also capable of shortening the onset of DNA laddering pattern (indication of apoptosis at early stage) by 6 hours and DNA laddering pattern peaked after 9 hours co-incubation with 3-AB. Morphologically, 3-AB did not switch cell death from necrosis to apoptosis; however, up to 30% of decrement in survived RBALC population could be observed after 12 hours repairing with 3-AB. These results suggested that 3-AB inhibited repairing of DNA single strand breaks induced by H2O2, it also might postpone cellular necrosis and redirected the cells to apoptosis by inhibiting PARP activities.

並列關鍵字

PARP ； 3-Aminobenzamide ； DNA single strand break ； Necrosis ； Apoptosis