摘要
To obtain a basal concentration of serum amyloid A (SAA) in cattle in Taiwan, SAA concentrations were determined from serum samples collected from 10 healthy heifers, once a week for 10 weeks. The values were also compared with those collected from 6 normal cows at the time of 4 weeks after parturition as well as 18 cows diagnosed with postpartum metritis. The purification and quantitative analysis of the serum SAA were performed by the methods of Sodium dodecyl sulfate-polyacryamide gel electrophroesis (SDS-PAGE) and Western blotting, followed by determining the optical densities of SAA on membranes using a densitometer. The determination of SAA concentrations developed in this study gave also a good correlation (r=0.914) with the commercial SAA ELISA kit. The SAA concentrations of 100 serum samples from the 10 heifers had a mean value of 22.3±1.1µg/ml, and there was no significant difference among the sampling months. No significant difference in SAA concentration was observed among the heifers during cold and warm seasons. The basal value of SAA in heifers was calculated as less than 43.7µg/ml. No significant difference in SAA concentration was observed among the 10 heifers during cold and warm seasons (21.9±1.9℃ vs 27.9±0.4℃). The mean serum SAA concentration from cows suffering from postpartum metritis was 85.4±9.0µ/ml, which was significantly greater than those of healthy heifers and postpartum cows (26.2±2.5µg/ml) (P＜0.01). These results demonstrate that SAA concentration may be a useful indicator for cows with inflammatory diseases.
並列摘要
To obtain a basal concentration of serum amyloid A (SAA) in cattle in Taiwan, SAA concentrations were determined from serum samples collected from 10 healthy heifers, once a week for 10 weeks. The values were also compared with those collected from 6 normal cows at the time of 4 weeks after parturition as well as 18 cows diagnosed with postpartum metritis. The purification and quantitative analysis of the serum SAA were performed by the methods of Sodium dodecyl sulfate-polyacryamide gel electrophroesis (SDS-PAGE) and Western blotting, followed by determining the optical densities of SAA on membranes using a densitometer. The determination of SAA concentrations developed in this study gave also a good correlation (r=0.914) with the commercial SAA ELISA kit. The SAA concentrations of 100 serum samples from the 10 heifers had a mean value of 22.3±1.1µg/ml, and there was no significant difference among the sampling months. No significant difference in SAA concentration was observed among the heifers during cold and warm seasons. The basal value of SAA in heifers was calculated as less than 43.7µg/ml. No significant difference in SAA concentration was observed among the 10 heifers during cold and warm seasons (21.9±1.9℃ vs 27.9±0.4℃). The mean serum SAA concentration from cows suffering from postpartum metritis was 85.4±9.0µ/ml, which was significantly greater than those of healthy heifers and postpartum cows (26.2±2.5µg/ml) (P＜0.01). These results demonstrate that SAA concentration may be a useful indicator for cows with inflammatory diseases.
延伸閱讀
- Chu, C. H. (2019). 偵測犬在偵測牛非臨床性乳房炎之應用 [master's thesis, National Pingtung University of Science and Technology]. Airiti Library. https://doi.org/10.6346/NPUST201900213
- 呂亞欣（2011）。台灣圈養鹿隻牛型結核病檢驗方法之評估〔碩士論文，國立屏東科技大學〕。華藝線上圖書館。https://doi.org/10.6346/NPUST.2011.00080
- 詹德裕（2011）。利用血液檢體偵測台灣牛隻及鹿隻的牛型結核病〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2011.01584
- Akinrinmade, J. F., & Akinrinde, A. S. (2012). Hematological, Serum Biochemical and Trace Mineral Indices of Cattle with Foreign Body Rumen Impaction. International Journal of Animal and Veterinary Advances, 4(6), 344-350. https://www.airitilibrary.com/Article/Detail?DocID=20412908-201212-201507210036-201507210036-344-350
- Allah, S. M. A., & Bakr, H. A. (2015). Serum Parathyroid Hormone Levels and Mineral Profiles in High Producing Dairy Cattle Around Calving Period. British Journal of Dairy Sciences, 4(1), 1-4. https://www.airitilibrary.com/Article/Detail?DocID=20442440-201501-201502240023-201502240023-1-4