虹鱒來源之鏈球菌（Lactococcus garvieae）感染途徑與巨噬細胞吞噬作用之相關性探討

The Relation between Infection Route of Lactococcus garvieae Originated from Rainbow Trout (Oncorhynchus mykiss) and Macrophage Phagocytosis

楊雨樵(Yu-Chiao Young) ； 王宏玲(Hung-Ling Wang) ； 陳媺玫(Meei-Mei Chen)

《臺灣獸醫學雜誌》 38卷2期 (2012/06) Pp. 128-137

https://doi.org/10.7009/TVJ.201206.0128

摘要

為探討鏈球菌之強弱毒株在虹鱒感染途徑之差異，以虹鱒來源之鏈球菌Lactococcus garvieae為模型，先經毒力活體試驗分辨出強毒株鏈球菌（R0726）與弱毒株鏈球菌（RT-2），將兩者之表面抗原經西方墨點法之分析，結果發現強、弱毒株之範型（Pattern）並無顯著差異。將強、弱毒株分別進行虹鱒之人工感染試驗，皆可在感染後兩小時於脾臟分離到菌體，但弱毒株在感染後18日已經分離不到鏈球菌，強毒株則直到感染後24日仍可分離到鏈球菌。在以虹鱒分離之巨噬細胞吞噬鏈球菌的實驗，結果顯示，強毒株除了在含抗體與補體之虹鱒血清中，於巨噬細胞內存活的菌數沒有顯著改變外，其餘狀況下被巨噬細胞吞噬之強毒株鏈球菌皆可存活且有顯著的胞內繁殖情形，而被吞噬之弱毒株鏈球菌則並未見到存活的狀況。

關鍵字

鏈球菌 ； 巨噬細胞 ； 虹鱒

並列摘要

In order to compare infection route between different virulence streptococcus strains, we have chosen Lactococcus garvieae originated from rainbow trout (Oncorhynchus mykiss) as an infection model. After in vivo virulence test, we found high virulence strain R0726 and low virulence strain RT-2. Surface antigen analyzed by western blotting and cannot find significant difference between two strains. After infection L. garvieae into rainbow trout, both strains could be isolated from spleen 2h post-infection. We cannot isolate RT-2 after 18th day post-infection, but after the 24th day post-infection we still can isolate R0726. Phagocytosed RT-2 were killed while R0726 significantly surviving within phagosomes of Macrophages.

並列關鍵字

macrophage ； rainbow trout ； streptococcus