The domestic large size TFT-LCD manufacturers invest capital to develop small size panel production gradually, but small size panel production has complex production process, so the important issues are to improve orders scheduling and bottleneck to minimize the makespan. The time of solving scheduling problem will depend on the problem size and it grows up with exponential increasing. When problem scope is smaller, it can be solved by general mathematical programming model to get the optimal solution. However, when the problem is bigger, it needs more time to have a solution. This study applied grouping technology to group similar type of processing first to reduce problem scope, and used ant colony optimization (ACO) method to solve the scheduling problems on bottleneck station.