九重葛黃化葉脈鑲帶病毒全長度基因序列之分析

Characterization of the full-length genome of Bougainvillea chlorotic vein banding virus

莊文馨(Chuang, W.-H.) ； 王怡瑾(Wang,Y.-J.) ； 王惠亮(Wang,H.-L.)

《植物醫學》 58卷3&4期 (2016/09) Pp. 127-132

https://doi.org/10.6716/JPM.201609_58(3_4).0003

摘要

於高雄市大樹區佛光山發現九重葛（Bougainvillea spectabilis）植株矮化和生長不良的病株，其葉片褪綠，沿葉脈出現黃化鑲帶（chlorotic vein-banding），及葉片捲曲等病徵。本研究取罹病葉片進行病毒純化。病毒形態呈桿菌狀（bacilliform），大小約為120-130×30nm。病毒具單片段基因體DNA，大小約8.7 kb。基因序列解析顯示其為九重葛黃化葉脈鑲帶病毒 （Bougainvillea chlorotic vein banding virus, BCVBV）。病毒之全長度基因組（accession number: EU034539）含8,759個核苷酸，具四個開放轉譯架構（ORFs 1, 2, 3, 和4）。BCVBV與12種Badnavirus屬病毒之全長度基因核苷酸序列相同度普遍偏低（36 - 45%）。

關鍵字

九重葛黃化葉脈鑲帶狀病毒 ； 桿菌狀DNA病毒屬 ； 青花菜嵌紋病毒科 ； 開放轉譯架構

並列摘要

Bougainvillea (Bougainvillea spectabilis) with symptoms of stunting and decline was discovered at Fuo Guang Shan in Kaoshiong. The affected leaves showed mottling, chlorosis, chlorotic vein banding, and leaf distortion. A virus was purified from diseased leaves and found to contain bacilliform virus particles of about 130 x 30 nm in size. The virus contains a monopartite genome of 8,759 nucleotides (accession number EU034539) and was identified as Bougainvillea chlorotic vein banding virus (BCVBV). The viral genome encodes 4 open reading frames (ORFs 1, 2, 3, and 4). The full length genome sequence of BCVBV displays relatively low nucleotide identities (36 - 45%) with those of 12 species of the Badnavirus.

並列關鍵字

Bougainvillea chlorotic vein banding virus ； Badnavirus ； Caulimoviridae ； open reading frame