芹菜細胞懸浮培養體胚誘導及植株再生最適條件之探討

Optimization of somatic embryogenesis and plantlet regeneration of celery (Apium graveolens L.) via suspension cell culture

廖珮妤(Pei-Yu Liao)

指導教授 ： 黃世佑

國立臺灣大學/工學院/化學工程學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.00340

摘要

植物體胚發生(somatic embryogenesis)為利用植物細胞所具之全潛能性(totipotency)，以體細胞誘導不定胚之技術。體胚細胞培養技術可應用於植物育種(交雜育種、轉殖)以及開發人工種子。本研究以芹菜(Apium graveolens L.)葉為培植體, 利用SH培養基(含2,4-D 7.5

關鍵字

人工種子 ； 芹菜 ； 密度梯度離心 ； 體胚發生 ； 植株再生

並列摘要

Due to totipotency of plant cells, somatic cells of plants inherently tend to produce vegetative propagation through somatic embryogenesis. The investigation of somatic embryogenesis motivates an agricultural breeding and a large-scale artificial seed system. The celery (Apium graveolens L.) was used as a model system to investigate the induction and cultivation conditions of embryo cells. At first, leaves were used to induce the callus (SH basal medium, 2,4-D 7.5

並列關鍵字

density gradient centrifugation ； celery ； artificial seed ； somatic embryogenesis ； plantlet regeneration

參考文獻

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