重組蛋白OraSE及GlmE最適化之生產條件探討

Optimization of the production conditions for recombinant proteins OraSE and GlmE

王立婷(Li-Ting Wang)

指導教授 ： 鄭建中

共同指導教授 ： 陳灝平(Hao-Ping Chen)

淡江大學/理學院/化學學系碩士班/碩士(2005年)

https://doi.org/10.6846/TKU.2005.00641

摘要

本研究藉由改變溫度的培養或使用不同的誘導因子，找出誘導生產蛋白質的最適化條件，以Eschericha coli合成的重組蛋白質OraSE及GlmE為探討對象。OraSE蛋白質可先後經由疏水性管柱及金屬螯合親和層析管柱分離，得到純的蛋白。另一蛋白質GlmE，也可經由疏水性管柱及陰離子交換管柱分離，達到純化的目的。並比較在不同溫度方式及誘導因子的培養下，所獲得蛋白質產量的差異。

關鍵字

純化 ； 濃度 ； 管住層析

並列摘要

Optimization of the production for recombinant proteins, OraSE and GlmE, was investigated by using several defferent growth conditions in this stusy . OraSE protein was purified by Phenyl – Sepharose HP and Ni2+ - NTA column ; GlmE protein was purified by Phenyl – Sepharose HP and Q – Sepharose FF. Significant difference in protein yield was observed when induction temperature and inducer were changed .

並列關鍵字

OraSE ； GlmE

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