大蒜水溶性化合物S-ethyl cysteine降低反式油酸造成小鼠氧化傷害之效應

Beneficial effects of garlic aqueous extract S-ethyl cysteine prevent the oxidative injury caused by elaidic acid in mice

黃子瑜(Zi-Yu Huang)

指導教授 ： 劉德中

中山醫學大學/健康管理學院/營養學系碩士班/碩士(2017年)

摘要

在先前的研究中已知大蒜水溶性化合物S-ethyl cysteine (SEC) 具有抗高血糖、抗心血管疾病、抗氧化等功能。本研究使用5週齡雄性C57BL/6小鼠隨機分為五組，在小鼠正常飲食下持續給予每週灌食2次，持續灌食8週，劑量為反油酸 (2 mL/kg B.W.) + 載劑芥花油 (6 mL/kg B.W.) 及芥花油組 (8 mL/kg B.W.)，並給予飲水添加S-ethyl cysteine (SEC) 1 g/L，每日紀錄其飲水量，於犧牲前12小時禁食後犧牲。結果顯示，在大蒜水溶性化合物SEC作用下，肝臟中抗氧化酵素有顯著提升，且肝損傷之情況有改善之趨勢，而血清內胰島素濃度及血糖濃度下降，因此推測大蒜水溶性化合物SEC具有降低反式油酸所造成之氧化傷害之功效。

關鍵字

大蒜水溶性化合物 ； SEC ； 反式油酸 ； 抗氧化 ； 肝損傷

並列摘要

We reported the protective effect of the garlic aqueous extract S-ethyl cysteine (SEC) previously, which has anti-hyperglycemia ,anti-cardiovascular disease and anti-oxidation function. In this study, 5 weeks old male C57BL/6 mice were randomly divided into five groups, and gavaged twice a week with calona oil (8 mL/kg B.W.) in calona oil group and elaidic acid oil (2 mL/kg B.W.) plus calona oil (6 mL/kg B.W.) in group and group EA+SEC, for eight weeks. In group SEC and group EA+SEC, we gave SEC 1g/L of water solution and record the water intake every day and than process NPO to 12 hours before sacrificed.
The data shows that after treatment with SEC, the anti-oxidation enzyme level were restored to the baseline, the liver injury situation was improved. In the serum, the insulin concentration and blood glucose level were decreased. As a result, we demonstrate that the garlic aqueous extract SEC can protect the oxidation injury of mice form trans fatty acid.

並列關鍵字

garlic aqueous extract ； SEC ； elaidic acid oil ； anti-oxidation ； liver injury

參考文獻

Aro, A., Jauhiainen M., Partanen R., Salminen I., Mutanen M. (1997). Stearic acid, trans fatty acids, and dairy fat: effects on serum and lipoprotein lipids, apolipoproteins, lipoprotein(a), and lipid transfer proteins in healthy subjects. Am J Clin Nutr 65:1419-26.

Barter, P., Gotto, A.M., LaRosa, J.C., Maroni, J., Szarek, M., Grundy, S.M., Kastelein, J.J., Bittner, V., and Fruchart, J.C. (2007). HDL cholesterol, very low levels of LDL cholesterol, and cardiovascular events. New England Journal of Medicine 357, 1301-1310.

Barnard, N.D., Bunner, A.E., and Agarwal, U. (2014). Saturated and trans fats and dementia: a systematic review. Neurobiology of aging 35, S65-S73.

Bordia, A., Verma, S., and Srivastava, K. (1998). Effect of garlic (Allium sativum) on blood lipids, blood sugar, fibrinogen and fibrinolytic activity in patients with coronary artery disease. Prostaglandins, leukotrienes and essential fatty acids 58, 257-263.

Chen, C.M., Yin, M.C., Hsu, C.C., and Liu, T.C. (2007). Antioxidative and anti-inflammatory effects of four cysteine-containing agents in striatum of MPTP-treated mice. Nutrition 23, 589-597.