植物生長調節劑對液體培養基蠔菇菌絲生長及出菇之影響

Effects of Plant Growth Regulators on the Mycelia Growth and Fruit Body Formation of Pleurotus ostreatus in Liquid Medium

邱獻毅(Sian-Yi Ciou) ； 趙苡蓁(Yi-Zhen Zhao) ； 張雅涵(Ya-Han Chang) ； 洪進雄(Chin-Hsiung Hung) ； 陳麗如(Li-Ru Chen)

《台灣農業研究》 66卷3期 (2017/09) Pp. 240-247

https://doi.org/10.6156/JTAR/2017.06603.07

摘要

本研究探討植物生長調節劑(plant growth regulator; PGR)在液體培養方式下對蠔菇菌絲、原基及子實體生長的影響。在分別添加1、6 mg L^(-1)的1-萘乙酸(α-naphthaleneacetic acid; NAA)、2,4-二氯苯氧乙酸(2,4-Dichlorophenoxyacetic acid; 2,4-D)或0.2、1.5 mg L^(-1)的6-苄氨基嘌呤(6-Benzylaminopurine; BA)的培養液中，接種蠔菇菌絲塊，進行25℃靜置培養。菌絲生長調查結果顯示，在添加1.5 mg L^(-1) BA或1、6 mg L-1 2,4-D的培養液中，菌絲生長量最高，在接種後21 d達7.5-7.8 cm，而6 mg L^(-1) NAA有抑制菌絲生長的情形。在原基及子實體形成的調查結果顯示，培養21 d後，在1 mg L^(-1) 2,4-D處理組中，原基及子實體形成數最多，其原基及子實體平均形成數分別為235.5個及5.2個。而在1.5 mg L^(-1) BA處理組中，原基形成數最低，平均為58.4個。在6 mg L^(-1) 2,4-D處理組中，子實體形成數最低，平均為2.6個。本研究顯示，培養液中添加1 mg L^(-1) 2,4-D能顯著影響蠔菇菌絲生長、原基誘導與子實體發生，未來可進一步進行放大試驗規模，尋求可以符合商業生產的植物生長調節劑及其最適濃度。

關鍵字

出菇 ； 液體培養 ； 植物生長調節劑 ； 蠔菇

並列摘要

The experiments were conducted to study the effects of plant growth regulators on the growth of mycelia and the formation of primordia and fruiting body of Pleurotus ostreatus cultured in liquid medium. The mycelia of P. ostreatus were inoculated in culture medium containing 1 mg L^(-1) or 6 mg L^(-1) α-naphthaleneacetic acid (NAA), 2,4-Dichlorophenoxyacetic acid (2,4-D), or 0.2 mg L^(-1) or 1.5 mg L^(-1) 6-Benzylaminopurine (BA) at 25℃ without shaking. The results showed that the growth of mycelia in liquid medium containing 1.5 mg L^(-1) BA or 1, 6 mg L^(-1) 2,4-D attained 7.57.8 cm after inoculation for 21 d. However, the treatment of 6 mg L^(-1) NAA inhibited the growth of mycelia. Twenty-one days after inoculation, treatment of 1 mg L^(-1) 2,4-D significantly induced the formation of primordia and fruiting bodies (P ＜ 0.05) with an avg. of 235.5 and 5.2, respectively. The application of 1.5 mg L^(-1) BA significantly reduced the number of primordia to the lowest (58.4 in avg.), and 6 mg L^(-1) 2,4-D treatment inhibited the formation of fruit bodies (2.6 in avg.). Study indicated that application of 1 mg L^(-1) 2,4-D in liquid medium can significantly enhance the growth of mycelia and the formation of primordia and fruit body of P. ostreatus. The scale-up experiments based on this study will be conducted for optimizing the commercial production of P. ostreatus in the future.

並列關鍵字

Fruit body formation ； Liquid culture ； Plant growth regulator ； Pleurotus ostreatus