表面消毒、培植體及植物生長調節劑對建立孤挺花組織培養小鱗莖繁殖之影響

Effect of Surface Disinfection, Explant Type, and Plant Growth Regulators on Establishment of In Vitro Bulblet Proliferation of Amaryllis

陳威臣(Uei-Chern Chen) ； 丁一(I. Din) ； 吳姿穎(Tzu-Ying Wu) ； 曹進義(Jhin-Yi Tsao) ； 夏奇鈮(Chi-Ni Hsia)

《台灣農業研究》 66卷4期 (2017/12) Pp. 286-297

https://doi.org/10.6156/JTAR/2017.06604.03

摘要

本研究以孤挺花(Hippeastrum hybridum Hort.)「紅獅」('Red Lion')、「千禧之星」('Blossom Peacock')及「台農1號-紅粉佳人」('Tainung No.1-Pink Lady')等3個品種之鱗莖為材料，探討消毒方法、培植體部位及生長調節劑組合對消毒效率與小鱗莖增殖之影響。結果顯示，「紅獅」短縮莖培植體 (2 cm × 1 cm × 1 cm)以1.2% NaOCl處理15 min之消毒效果較佳，且以靜置隔夜處理相對於直接處理的汙染率較低。比較「台農1號」上半部與下半部短縮莖培植體(6 mm × 6 mm × 2 mm)之消毒結果顯示，上半部培植體不僅汙染率低且小鱗莖誘導率較高。利用「千禧之星」小鱗莖(周徑約2.5 cm)之1/2縱切或1/4縱切鱗莖作為培植體進行試驗，1/4鱗莖處理之每個鱗莖可形成5.2個小鱗莖，顯著高於1/2鱗莖處理之2.0個小鱗莖。「紅獅」1/4鱗莖培養於1-4 mg L^(-1)苯甲基腺嘌呤(benzylaminopurine; BA)或0.1-0.4 mg L^(-1)賽本隆(thidiazuron; TDZ)配合0.2 mg L^(-1)奈乙酸(α-naphthalene acetic acid; NAA)之MS培養基，結果顯示0.1 mg L^(-1) TDZ處理可獲得較高之小鱗莖增殖率，經8 wk培養後共獲得11.6個小鱗莖。本研究結果可提供新品種孤挺花在種苗量化參考。

關鍵字

朱頂紅 ； 消毒 ； 離體繁殖 ； 賽本隆

並列摘要

Field-grown bulbs of Amaryllis (Hippeastrum hybridum) 'Red Lion' (about 25 cm circumference), 'Blossom Peacock', and 'Tainung No. 1-Pink Lady' (about 12 cm circumference) were used as culture materials. Effects of surface disinfection, explant type, and plant growth regulators combinations on establishment in vitro bulblet proliferation were investigated. Stem explants (2 cm × 1 cm × 1 cm) of 'Red Lion' left on a filter paper overnight before using 1.2% sodium hypochlorite solution for 15 min resulted in the least contamination. Stem explants (6 mm × 6 mm × 2 mm) cut from the upper or lower part of 'Tainung No. 1-Pink Lady' were compared. Although explants derived from different positions of the stem had the same proliferation rates of 1.2 bulblets per explant, the upper stem explants had a significant lower contamination rate of 3% than that of 37% from the lower part explants. Comparison of proliferation efficiencies was conducted using half- and quarter-bulb explants of 'Blossom Peacock' derived from an in vitro bulb with 2.5 cm circumference in size, and no significant difference was found on bulblet formation between both treatments. However, a higher proliferation rate of 5.2 bulblets per original bulb was calculated by using quarter-bulb explants than that of 2.0 bulblets from the half-bulb explants. The highest bulblet proliferation rate with 11.6 bulblets from per 2.5 cm circumference in 'Red Lion' in vitro bulb was obtained by culturing quarter-bulb explants on the MS medium supplemented with 0.1 mg L^(-1) thidiazuron (TDZ) and 0.2 mg L^(-1) α-naphthalene acetic acid (NAA) for 8 wk. Results of this study could provide useful information on bulblet proliferation for new varieties of Amaryllis.

並列關鍵字

Hippeastrum hybridum ； Disinfection ； In vitro propagation ； Thidiazuron