Background and Purpose: Shoulder injuries are common in overhead athletes due to the demand of the repetition and high velocity movements. After enduring an injury, returning to play (RTP) at the same level of performance is the critical issue. However, studies showed that the decision making of RTP is so complicated and there’s a lack of objective measurements for RTP especially for upper extremity injuries. The objective of this study was to characterize functional performance in symptomatic overhead athletes. Methods: Overhead athlete ranges from 18 to 45 year-old with shoulder injury would be recruited in this study. According to the Kerlan-Jobe Orthopaedic Clinic Shoulder and Elbow (KJOC) Questionnaire, the participants would be allocated into playing but with arm trouble and not playing due to arm trouble. The functional performance was assessed by KJOC Questionnaire score, touches of Closed Kinetic Chain Upper Extremity Stability Test (CKCUEST) and ratio of Unilateral Seated Shot Put Test (USSP) in dominant and non-dominant hands randomly. The difference between 2 groups would be identified. Results: Nine symptomatic overhead athletes were allocated into playing group (n = 5) and not playing group (n = 4). Considering the length of injury as covariate, there were no significant differences between groups in KJOC (p = 0.244), CKCUEST (p = 0.625), and USSP (p = 0.855). The post-hoc power analysis showed that type II error was likely as small power (0.072 and 0.053) and moderate effect size (0.628 and 0.347) on CKCUEST and USSP, respectively. Conclusion: Larger sample size was needed to validate whether there was functional performance difference between playing and not playing in symptomatic overhead athletes. Clinical Relevance: Functional performance assessments seem to be potentially used to characterize playing or not playing in symptomatic overhead athletes.