Journal of International Dental and Medical Research
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Ectodermal Dysplasia Group-Turkey,正常發行
選擇卷期
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Evidence suggests that several genes; including Myo1H, play an important role in the etiology of Class III malocclusion. Single nucleotide polymorphism (SNP) in marker rs10850110 (locus 12q24.11) within Myo1H gene has been associated with the incidence of mandibular prognathism (MP). MYO1H is a class 1 myosin which has been implicated in various motile processes including cytoskeleton reorganization. Therefore, genetic alteration in genes responsible for muscle function will also affect the skeletal growth. This study aimed to detect the presence of Myo1H (rs10850110) SNP and to determine its genotype and allele distribution in MP patient in the local population. The sample comprises of 31 patients; 14 patients from class I malocclusion (control samples) and 17 patients from class III malocclusion (MP). Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for the marker rs10850110 and the genotyping was done by sequencing. Chi-square test was used to determine the over-representation of marker allele (p＜0.05). Presence of Myo1H SNP (rs10850110) was detected in local population analysed and the distribution of its genotype and allele could be observed. There were significant differences between allele (p=0.000) and genotype (p=0.000) frequency within and between control (Class I) and Class III malocclusion. Our findings are in agreement with previous studies suggesting positive influence of Myo1H (rs10850110) SNP in the incidence of MP. Further studies should be developed in order to understand the exact role and mechanism of Myo1H in different classes of malocclusions.
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The prevalence of Recurrent Aphthous Ulcer (RAU) is varied in different regions of the world. There is insufficient survey in prevalence of RAU in Malaysia as the only latest figure available is about 18 years ago. This study aimed to measure the prevalence of RAU in patients attending Dental Clinic-college of dentistry within 5 academic years and to know the distribution and relation of RAU based on the most common type of RAU, gender, race and age range. A retrospective cross-sectional study based on the confirmed case of RAU from the students' Oral Medicine logbooks and case sheets from the attended patients’ folder to Oral Medicine Dental Clinic- college of dentistry from academic year 2012/2013 to 2016/2017. 160 patients were diagnosed of RAU.0.42% of prevalence among the total patients was found to have RAU. As compared between the Oral Medicine cases, patients that were diagnosed with RAU shows prevalence of 11.13%. The most common type was minor RAU (96.9%), followed by major (3.1%) and no patient was presented with herpetiform type. Based on gender, females showed more predilection of RAU compared to males with 60.6%. Age range of 20-29 showed the highest percentage of 71.3 % and Malay showed the most common race of RAU compared to Chinese and Indian which was 97.4%. The results indicate there is a relation between the type of RAU, age, gender, race and with the prevalence of RAU. Further studies should include larger sample size with a wider range of populations.
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Antiseptic Effect of Betel Quid Extract on Lip Mucosal Wound of male Wistar (Rattus novergicus) Rats
Antiseptic is commonly used in dentistry practices, but some antiseptics have side effects such as allergy. Therefore, natural substances are developed as substitute, i.e betel quid which consisted of betel leaf, areca nut, gambier, and mineral slaked lime. The aim of this study was to determine antiseptic effect of betel quid extract on lip mucosal wound of male Wistar (Rattus norvegicus) rats. This study was true experimental in vivo. Thirty rats were divided into five groups; three groups were treated with betel quid extract ointments at concentration of 5%, 10% and 20%, one group was positive control (hyaluronic acid) and last group was negative control (placebo). One-mm diameter of wound was made on lower lip mucosa of rats with cylinder diamond bur. All wounds were swabbed before and after treatment, and the number of bacterial colonies grown in agar plates were counted. The results showed that there was significant decrease of bacterial colony numbers for respective groups, except for negative control (p ＜ 0.05). Ointment containing 20% betel quid extract had antiseptic effect similar to 0.2% hyaluronic acid. It can be concluded that betel quid extract had antiseptic effect on lip mucosal wound.
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Objective of this study is to investigate the effect of daily consumption of Lactobacillus reuteri ATCC 55730 containing probiotic lozenges on salivary Streptococcus mutans serotype c, Streptococcus sobrinus, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans number, in patients undergoing orthodontic treatment with fixed appliance. Saliva subject collected before and two weeks after daily probiotic consumption. S. mutans serotype c, S. sobrinus, P. gingivalis ATCC 33277, and A. actinomycetemcomitans ATCC 29522 were cultured in BHI-broth (anaerobic-condition). After 48-h incubation, the number of colonies on each dilution plate were used to extrapolate a standard curve. Bacterial DNA were extracted using heat-shock method. Subsequently, quantitative Real-Time PCR method was applied to analyse the number of S. mutans serotype c, S. sobrinus, P. gingivalis, and A. actinomycetemcomitans in saliva. Total number of DNA target were identified using SYBR Green (Applied Biosystem) and 16S rRNA gene specific primers for each bacterium tested. The research results showed that there was significant difference in the average scores of S. mutans serotype, S. sobrinus, P. gingivalis, and A. actinomycetemcomitans before probiotic consumption compared to two weeks after probiotic consumption. The results show that probiotic L. reuteri ATCC-55730 brought a statistically significant reduction of S. mutans serotype c, S. sobrinus, P. gingivalis, and A. actinomycetemcomitans after two weeks consumption of probiotic in fixed orthodontic patients (P＜ 0.05). It is concluded that L. reuteri ATCC 55730-containing probiotic lozenges consumption reduces the number of S. mutans serotype c, S. sobrinus, P. gingivalis, and A. actinomycetemcomitans in saliva subjects during fixed orthodontic appliances.
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To determine the effects of Bilimbi fruit leaf (BL) extract on the corrosion rate of stainless steel orthodontic wires. A total of 60 stainless steel orthodontic wires (0.7 mm in diameter) were divided randomly into 4 groups (n=15): negative control group (control), 3% Bilimbi fruit leaf extract (B03), 6% Bilimbi fruit leaf extract (B06), and 10% Bilimbi fruit leaf extract (B10). Samples were immersed in artificial saliva and incubated at a temperature of 37°C for 7 days. Subsequently, the rate of corrosion was determined using a weight loss measurement method. The data were then analyzed using one-way analysis of variance (ANOVA) followed by least significant difference (LSD) post hoc test (P ＜ 0.05). One-way ANOVA showed a significance P-value of ＜ 0.05 (P = 0.000). Post-hoc LSD test also showed that there was a significant difference between control and treatment groups, in which the group immersed in 10% of BL extract had the lowest corrosion rate compared to the other two experiment groups. In addition, the 10% BL extract group had the highest percentage inhibition efficiency compared to the other two groups (79.84%). It can be concluded that Bilimbi fruit leaf extract can inhibit corrosion of stainless steel orthodontic wires. Ten percent of BL extract demonstrated a greater corrosion inhibition efficiency compared to 3% and 6% of the extract.
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The objective of this study was to investigate the growth of Candida albicans on acrylic resin plate after soaking in freshly squeezed garlic. Samples were immersed in sterile saliva for 1 hour at room temperature for the formation of pellicle. They were taken and rinsed with phosphate buffer saline (PBS) solution, contaminated with Candida albicans by insertion into a tube containing Candida albicans suspension, incubated for 24 hours at 370 C, removed and rinsed with PBS solution, soaked in freshly squeezed garlic in test tubes for 3 minutes, containing 10 ml of freshly squeezed garlic of 20%, 30 %, 40 % concentration each and sterile distilled water. Samples were taken and rinsed with PBS, inserted into Sabouraud's broth, vibrated with vortex for 30 seconds to release Candida albicans attached to them. Suspension from Sabouraud's broth was taken, dripped on Sabouraud's dextrose agar, spreading, incubated for 48 hours, at 370C. Candida albicans colonies were calculated. Data were analysed using Anova and followed by LSD test. The Results showed that there were significant difference among the groups (p ＜ 0.05). In conclusion, the higher concentration of freshly squeezed garlic the lower the number of Candida albicans colonies.
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Previous study reported that plant natural product promotes stem cell growth. Flaxseed (Linum usitatissimum) contains numerous compounds recognized for its health benefits. This study aimed to investigate the biological effects of flaxseed crude extract on stem cells obtained from human exfoliated deciduous teeth (SHED). Whole flaxseeds were ground and extracted with absolute ethanol using soxhlet extractor. The effects of flaxseed crude extract on SHED were assessed at concentration 1, 2, 4, 8 and 16 mg/ml for cell viability using MTT assay, cell morphology using inverted microscope and proliferative activity describe as population doubling time (PDT) using alamarBlue assay. The fatty acid composition of flaxseed was analysed using gas chromatographymass spectrometry (GCMS) instrumental technique.Although insignificant, flaxseed at concentration up to 4 mg/ml slightly increased SHED proliferation activity while maintaining cell viability and morphology. However, 8 mg/ml of flaxseed inhibited cell viability and proliferation activity, and changed the cell morphology. GCMS analysis revealed the presence of linolenic acid, linoleic acid, palmitic acid and stearic acid. Overall, ethanolic crude extract of flaxseed at concentration up to 4 mg/ml slightly enhanced the growth and maintained the morphology of SHED.
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Honey has been shown to promote tissue healing and used to cure many diseases since ancient times. Nevertheless its effect on periodontal therapy has very limited evidence. The aim of this study was to evaluate the potential complementary effect of Tualang honey applied in adjunct to periodontal therapy. Fourteen chronic periodontitis patients with total number of 94 sites with periodontal pocket of 5mm and above were randomly assigned into control and test groups. Parameters such as plaque score, gingivitis score, pocket depth and clinical attachment loss were recorded. Scaling and root planing were done in all patients followed with honey application into the periodontal pockets of test group. The parameters were reevaluated after six weeks. The periodontal pocket depth and clinical attachment loss were significantly improved after 6 weeks in both groups (P＜.05). However, the magnitude of changes in all parameters showed no significant different between control and honey group. Considering limitation of this study, the superior effect of Tualang honey as an adjunct to nonsurgical periodontal therapy was not evidence. Many factors may involve the sustainability of honey in the periodontal pocket. Thus we recommended further investigation to evaluate the effect of honey on periodontal wound healing.
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To analyze compounds of dental enamel after strawberry gel application. This study using six central incisors maxillary that has been extracted. Six central incisors maxillary were divided into five time series, one tooth is used as a control and five tooth given applications of strawberry gel for 1 hour, 1 hour 30 minutes, 2 hours, 2 hours 30 minutes and 3 hours. Then, compounds of dental enamel observed by using Energy-Dispersive X-Ray Spectroscopy. An analysis using nonparametric test (Kruskal Wallis test) to determine differences compounds that occur at each time range. The results of this study, p value = 0.406＞ 0.05. This means there is no significant difference on dental enamel compound between the control group and the group that had been treated with strawberry gel application. There was no significant effect between time duration and changes in mineral compounds of dental enamel after application of strawberry gel.
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Indonesia with a large area, the population of more than two hundred million people, spread over several islands, so it is estimated to have different behavior to determine the place, the cost for dental health care. Purpose: The purpose of this research is to know the behavior of dental health care for Indonesian people, by using telephone survey. Method: A sample of 180 is a known cell phone user from a Telkomsel provider (Cellular Telecommunication) is divided into 6 regions representing regions in Indonesia. Samples of each region were randomly selected systematically, then contacted by telephone for interviews. long interview no more than 15 minutes. Results: The results showed the average percentage of visits to dental care centers last year was 38.3%. Selecting dental care in private dental practice is 53.9%, choosing services in Public Health Centers are 30% and hospitals are 16.11%. The cost of treatment sourced from personal funds was 58.9%, using of insurance (BPJS is 41,1%. Conclusion: The Indonesian people's behavior toward regular dental care every year is still low, and the use of facilities in Public Health Centers has not been maximized.