Development of a Lateral-Flow Assay for Rapid Screening of the Performance-Enhancing Sympathomimetic Drug Clenbuterol Used in Animal Production; Food Safety Assessments
Wei-Hua Lai；Yang Xu；Daniel Y. C. Fung；Yong-Hua Xiong
lateral-flow ； strip ； screen ； clenbuterol
Asia Pacific Journal of Clinical Nutrition
|Volume or Term/Year and Month of Publication||
16卷S1期（2007 / 04 / 01）
106 - 110
A lateral-flow assay that could provide visual evidence of the presence of clenbuterol in swine urine was developed. Colloidal gold was prepared and conjugated with anti-clenbuterol monoclonal antibody. Immunochromatographic test strips were produced, and then, 210 samples were tested on these strips. Analysis was completed in 10 min. Detection limit was 3 ppb of clenbuterol. Parallel GC-MS data indicated that clenbuterol rapid detection strip had no false negative. The false positive rate was 4.4%. Immunochromatographic strip has great applied value in the food safety field because it possesses benefits of sensitivity, stability, reproducibility, ease of use and inexpensive.