Curcumin Protects Mitochondria from Oxidative Damage and Attenuates Apoptosis in Cortical Neurons
Yuan-Gui Zhu；Xiao-Chun Chen；Zhi-Zhe Chen；Yu-Qi Zeng；Guang-Bin Shi；Yan-Hua Su；Xu Peng
Curcumin ； tert-butyl hydroperoxide ； apoptosis ； oxidative stress ； Bcl-2 family proteins ； mitochondria ； Alzheimer disease
Acta Pharmacologica Sinica
|Volume or Term/Year and Month of Publication||
25卷12期（2004 / 12 / 01）
1606 - 1612
AIM: To investigate the effect of curcumin on tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in rat cortical neurons and to explore the possible mechanism. METHODS: Primary cultured rat cortical neurons were performed in vitro and cell viability was measured by MTT assay. DNA fragmentation was used to evaluate cell apoptosis. Intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨm) was determined by flow cytometric assay. Cellular glutathione (GSH) content was measured by spectrophotometer: Bcl-2 family proteins, cytochrome c, cleaved caspase-3, and poly (ADP-ribose) polymerase (PARP) were detected by Western blot. RESULTS: Exposure of tBHP 100μmol/L to neurons for 60 mim resulted in ΔΨm loss and cytochrome c release from mitochondria and subsequent activation of caspase-3 and PARP cleavation, and cell apoptosis. After removal of tBHP and then further treatment with curcumin (2.5-20μmol/L) for 18 h, curcumin abrogated ΔΨm loss and cytochrome c release, blocked activation of caspase 3, and altered the expression of Bcl-2 family. Further curcumin treatment also prevented cellular GSH and decreased intracellular ROS generation markedly. Curcumin eventually attenuated tBHP-induced apoptosis in cortical neurons. CONCLUSION: Curcumin may attenuate oxidative damages in cortical neurons by reducing intracellular production of ROS and protecting mitochondria from oxidative damage.