Evaluation of antibiotic activity produced by Pseudomonas aeruginosa LV strain against Xanthomonas arboricola pv. pruni




Fernanda Corrêa da Silva Vasconcellos;Admilton Gonçalves de Oliveira;Lucilene Lopes-Santos;Jamile Priscila de Oliveira Beranger;Martha Viviana Torres Cely;Ane Stefano Simionato;Juliana Fonteque Pistori;Flavia Regina Spago;João Carlos Palazzo de Mello;Juca Abramo Barrera San Martin;Celia Guadalupe Tardeli de Jesus Andrade;Galdino Andrade

Key Words

Peach Tree ; Bacterial Spot ; Biological Control ; Leaf Lesions ; Xanthomonas arboricola Pv. pruni


Agricultural Sciences

Volume or Term/Year and Month of Publication

5卷1期(2014 / 01 / 01)

Page #

71 - 76

Content Language


English Abstract

Bacterial spot caused by Xanthomonas arboricola pv. pruni (Xap) is considered as a major problem in peach orchards. Copper and antibiotics are used to control, and biocontrol should be a new alternative with low environment impact. The objective was evaluated by the antibiotic activity of the metabolite produced by Pseudomonas aeruginosa LV strain. The free cells supernatant was fractionated with a serial organic solvent with crescent polarity and a dichloromethane phase was concentrated and lyophilised, and after was fractionated using vacuum liquid chromatography. The antibiotic activity of the F3 fraction containing an organometallic compound was tested on Xap in vitro and in a greenhouse conditions. Plants were sprayed with F3 before or after Xap infection and the results showed changes in exopolysaccharides and cell morphology. The F3 concentration of 450 μg.mL^(-1) was more effective. The results showed that F3 fraction could be a new alternative to control bacterial spot.

Topic Category 生物農學 > 農業
生物農學 > 森林
生物農學 > 畜牧
生物農學 > 漁業