培養條件對乳酸菌胞外多醣生產及抗氧化性之影響

Effect of fermentation conditions on production and antioxidative activity of exopolysaccharide from several lactics

陳智強(Chih-Chiang Chen)

指導教授 ： 游若篍

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.02443

摘要

本試驗探討不同碳源、培養溫度及起始pH 值等條件，對乳酸菌胞
外多醣生合成之影響。此外，並分析以不同碳源培養所生成之胞外多
醣之抗氧化活性。結果顯示葡萄糖較有利於Lactobacillus acidophilus
BCRC 14079、L. bulgaricus BCRC 10696、L. plantarum BCRC 10069、
Leuconostoc mesenteroides BCRC 12273 生產胞外多醣，產量為400-500
mg/L。蔗糖有利於L. rhamnosus GG BCRC 16000 胞外多醣之生產，胞
外多醣產量為500 mg/L。Streptococcus thermophilus BCRC 14085 則以
乳糖為碳源，可產生較多量之胞外多醣，為400 mg/L。L. bulgaricus
BCRC 10696、L. plantarum BCRC 10069、L. rhamnosus GG、L.
mesenteroides BCRC 12273、S. thermophilus BCRC 14085 在25℃培養之
胞外多醣產量高於37℃。L. acidophilus BCRC 14079 則於37℃培養之
產量較高。六株乳酸菌於起始pH 值為6.5 環境下之胞外多醣產量，皆
高於起始pH 值為4.5 之環境。所有菌株以葡萄糖、乳糖、蔗糖為碳源
合成之胞外多醣，具有清除DPPH 自由基、超氧陰離子、過氧化氫及
羥基自由基之能力，亦可螫合亞鐵離子，並有些微還原力，而以L.
plantarum BCRC 10069 生產之胞外多醣清除羥基自由基的效果最佳，為64％。

關鍵字

抗氧化 ； 乳酸菌 ； 胞外多醣

並列摘要

In this study, we investigated the effect of fermentation conditions such
as carbon source requirement, temperature and initial pH value on the
production of exopolysaccharides（EPS）by several lactic acid bacteria. The
antioxidative activities of exopolysaccharide from lactics grown on various
sugars were also analyzed. The results indicated that after 48 h of
incubation, glucose was a better carbon source for Lactobacillus
acidophilus BCRC 14079, L. bulgaricus BCRC 10696, L. plantarum
BCRC 10069, and Leuconostoc mesenteroides BCRC 12273 to produce
exopolysaccharide, and the yields of EPS were between 400 to 500 mg/L.
Sucrose was a better carbon source for L. rhamnosus GG BCRC 16000 to
produce EPS, and the amount of EPS was 500 mg/L. Streptococcus
thermophilus BCRC 14085 produced more exopolysaccharide from lactose,
and the yield was 400 mg/L. The higher yield of exopolysaccharide from L.
bulgaricus BCRC 10696, L. plantarum BCRC 10069, L. rhamnosus GG
BCRC 16000, L. mesenteroides BCRC 12273, and S. thermophilus BCRC
14085 was obtained while cultivation at 25℃ than at 37℃. However, the
yield of EPS from L. acidophilus BCRC 14079 cultivation at 37℃ was
more than that cultivation at 25℃. All selected LAB strains using a
medium with initial pH 6.5 showed more EPS yield than those with initial
pH 4.5. The exopolysaccharide produced by all LAB strains cultivation
with glucose, lactose or sucrose showed scavenging effect on DPPH
radicals, superoxide anion, hydrogen peroxide, and hydroxyl radicals. The
EPS also showed chelating ability on ferrous ion and some reducing power.
Among all the exopolysaccharide produced by selected lactics, the EPS
from L. plantarum BCRC 10069 showed the greatest scavenging activity,
64％, against hydroxyl radicals.

並列關鍵字

antioxidative activity ； exopolysaccharide ； lactic acid bacteria

參考文獻

degenerative diseases of aging. Proc Natl Acad Sci USA 90: 7915-7922.

AOAC. 1984. Official Methods of Analysis of the Association of Official

Analytical Chemists. Washington, DC, USA.

S. 1992. Extracellular polysaccharide from encapsulated Streptococcus

J Food Sci 57: 625-628.