Translated Titles

Effects of Chitosan on Post Bioceramic Coated Ti6Al4V Implant Alloy



Key Words

幾丁聚醣 ; 骨細胞 ; 增殖 ; 分化 ; chitosan ; osteoblast ; proliferation ; differentiation



Volume or Term/Year and Month of Publication


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Content Language


Chinese Abstract

本研究企圖將幾丁聚醣(chitosan)塗層沈積於已被覆生醫陶瓷之Ti6Al4V上。希望藉由chitosan可被人體吸收、能吸附蛋白質的特性,增加植入材的生物活性,促進骨細胞貼附、增殖與分化的能力。經由XRD、SEM/EDS、AFM、拉伸附著力實驗、陰極極化實驗、浸泡實驗、體外骨細胞培養實驗等探討各種塗層特性。結果顯示, chitosan可以深入HT鍍層的空隙,大幅降低鍍層的表面粗糙度並且增加HA鍍層在拉伸實驗後仍然存在試片表面的比例。由於chitosan擁有許多胺基與氫氧基能吸附模擬體液中的鈣、磷酸根離子的特性,可增加CHT鍍層的生物活性。此外chitosan能避免塗層產生龜裂並提高抗腐蝕性而減少有害人體的金屬離子釋出。體外骨細胞培養實驗包括MTT與ALP試驗顯示chitosan能進一步提升骨細胞在生醫陶瓷塗層的增殖與分化。

English Abstract

To improve the bioactivity, attachment, proliferation and differentiation of osteoblast, the deposition of chitosan was conducted on post biceramics coated Ti6Al4V implant alloy. In the study, the coated specimens were characterized by XRD, SEM/EDS analysis, AFM, adhesion test, electrochemical polarization tests, immersion tests and cell culture. The results showed that chitosan can pentrate and fully cover the porous bioceramics coating and finally result in the decrease of surface roughness. Immersion tests showed that chitosan could improve the bioactivity of coating, avaid the formation of the cracks, raise the corrosion resistance and reduce the metal ion release. The in vitro cell culture including MTT and ALP also revealed the better proliferation and differentiation of osteoblast on chitosan coated specimen.

Topic Category 工學院 > 材料科學與工程學系所
工程學 > 工程學總論
  1. 【1】Shih, C.H., Lee, P.C., Chen, J.H., Tai, C.L., Chen, L.F., Wu, J.S.S., Chang, W.H., 1997. Measurement of Polyethylene Wear in Cementless Total Hip Arthroplasty. Journal of Bone and Joint Surgery. British Volume 79, pp. 361-165.
  2. 【3】Maloney W.J. et al, Trans ORS, 68,1996.Chemotaxis and Activation of Particle-Challenged Human Monocytes in Response to Monocyte Migration
  3. Inhibitory Factor and C–C Chemokines
  4. 【5】K. Merritt, S. A. Brown, J. Biomed. Mater. Res., 22, 111, 1998.
  5. 【7】S. A. Brown, “In vitro and in vivo metal ion release,” J. of Biomed. Mater. Res., 22, P.321-338 (1988)
  6. 【9】E. Merian, “Metalle in der Umwelt: Verteilung, Analytik and biologische Relevanz,” Verlag Chemie, Weinheim.
  7. 【10】G. Macara “Vanadium, an element in search of a role,” Trends Biochem. Sci., 5, P.92-95 (1980)
  8. 【14】J. J. Callaghan, (1993) "Current concepts review: The clinical results and basic science of total hip arthroplasty with porous-coated prostheses", J. Bone Joint Surg., 75A299-310.
  9. 【15】M. C. Sunny, (1991) “Titanium-Protein interaction: charges with oxide layer thickness,” Journal of Biomaterials Applications, 6, 89-98.
  10. osseointegrated implants in the treatment of the edentulous jaw” Int J Oral Surg, P.387–461
  11. 【19】Leonor, I.B., (2003) “In vitro bioactivity of starch thermoplastic /hydroxyapatite composite biomaterials: an in situ study using atomic force microscopy”, Biomaterials, 24, P.579-585
  12. 【20】Kim, Hae-Won., (2004) “Hydroxyapatite coating on titanium substrate with
  13. for hydroxyapatite coating on titanium substrate”, Biomaterials, 25,P.4393-4403
  14. deposition (IBAD) of hydroxyapatite coating layer on Ti-based metal
  15. substrate”, Biomaterials, 21, P. 469-473
  16. 【24】Hae-Won Kim,(2004), “Hydroxyapatite coating on titanium substrate with
  17. 【25】Kokubo, Tadashi., (1998) “Apatite formation on surfaces of ceramics, metals
  18. and polymers in body environment”, 46, P. 2519-2527
  19. 【27】American Ceramic Society, (1983). "Phase Diagrams for Ceramists", Vol. 5,
  20. pp.321-322, American Ceramic Society, Washington DC.
  21. 【30】ASTM F-67, “Standard Specification for Unalloyed Titanium for Surgical Implant Applications”, Annual Book or ASTM Standards, American Society for Testing and Materials, Vol. 3.01
  22. 【33】ASTM F-90, “Standard Specification for Wrought Cobalt-20 Chromium-15 Tungsten-10 Nickel Alloy for Surgical Implant Applications”, Annual Book or ASTM Standards, American Society for Testing and Materials, Vol. 3.01
  23. 【34】J. Ma. Rincon, M. Romero, (1999), “Characterization techniques of glasses and ceramics”, New York,
  24. 【35】L. Jianguo, (1993), “Behavior of titanium and titania-based ceramics in virto and in vivo”, biomaterials, 14, P.229-232
  25. 【37】蘇建榮, (2002),針對不同表面處理之鈦合金骨螺絲做生物親合姓的評估,國立成功大學醫學工程研究所碩士論文,.
  26. 【38】Jarcho M. 1981, Calcium phosphate ceramics as hard tissue prosthetics. Clinic Orthop, 157:259
  27. 【39】Thomas K A, Kay J F, Cook S D, et al. 1987,The effect of surface macrotexture and hydroxyapatite coating on the mechanical strengths and histologic profiles of titanium implant materials. J Biomed Mater Res, 21:1395
  28. 【40】Chen J, Wolke J G C, de Groot K.1994,. Microstructure and crystallinity in hydroxyapatite coatings. Biomaterials, 15:396
  29. 【41】Chen J, tong W, Cao Y, et al. 1997, Effect of atmosphere on phase transformation in plasma sprayed hydroxyapatite coatings during heat treatment. J Biomed Mater Res, 34:15
  30. 【42】Sergey V. Dorozhkin & Matthisa Epple, (2002). “Biological and medical significance of calcium phosphates.”, Angew. Chem. Int. Ed., 41, 3130-3146
  31. 【43】F G. Daculsi, J. -M. Bouler, and R. Z. Legerost, (1997). “Adaptive crystal formation in normal and pathological calcifications in synthetic calcium phosphate and related biomaterials,” International Review of Cytology, 172, 129-191
  32. 【47】Gibbon D F.Tissue response to resorbable synthetic polymers.In:Planck H ed. 1992, Degradation Phenomena on Polymeric Biomaterials.
  33. Germany:Springe-Verlag Berlin Heidelberg, 97~104
  34. 【48】Menei P Daniel V, Benoit J P. 1993. Biodrgradation and brain tissue reaction to poly(DL-lactide-co-glycolide)micropheres. Biomaterials ,14:470~478
  35. 【53】Muzzarelli RA, Biagini G, Bellardini M, Simonelli L, Costaldini C, Fratto G. 1993. Osteoconduction exerted by methylpyrrolidinone chitosan used in dental surgery. Biomaterials;14:39 – 43.
  36. 【54】Muzzarelli RA, Mattioli-Belmonti M, Tiez C, Biagini G, Ferioli G, Brunelli MA. 1994. Stimulatory effect on bone formation exerted by a modified chitosan. Biomaterials;15:1075–1081.
  37. 【55】Muzzarelli RA, Zucchini C. Ilari P, Pugnaloni A, Mattioli-Belmonti M, Biagini G. 1993. Osteoconductive properties of methylpyrrolidinonechitosan in an animal model. Biomaterials;14:925–929.
  38. 【56】Malette WG, Quigley HJ, Adickes ED. 1986. Chitosan effect in vascular surgery, tissue culture and tissue regeneration. In: Muzzarelli R, Jeuniaux C, Gooday GW, editors. Chitin in nature and technology. New York: Plenum Press; p435– 442.
  39. 【57】Fradet G, Brister S, Mulder DS, Lough J, Averbach BL. 1986. Evaluation of chitosan as a new hemostatic agent: In vitro and in vivo experiments. In: Muzzarelli R, Jeuniaux C, Gooday GW, editors. Chitin in nature and technology. New York: Plenum Press; p 443–451.
  40. 【62】Ghaouth, A E., Arul, J., Grenire, J., and Asselin, A. 1992. Antifungal activity of chitosan on two postharvest pathogens of strawberry fruit. Phytopathology. 82:398-402
  41. 【69】Sudarshan, N. R.; Hoover, D. G.; Knorr, D. (1992), Antibacterial action of chitosan. Food Biotechnol 6, 257–272
  42. 【70】Kurosawa H, Aoki H, Akao M.(1986) , Tissue reaction to calcinated bone graft. Biomaterials7:132
  43. 【71】Allen J. Bard, Larry R. Faulkner.,(2001) “Electrochemical methods, fundamentals and applications”, New York, Wiley.
  44. 【72】Olof Forsen.,(1992) “Electrochemical methods in corrosion research”, Switzerland, Trans Tech.
  45. 【75】I. Zhitomirsky, (1998), “Cathodic electrosynthesis of titanium and ruthenium oxides”, Materials Letters, 33, P.305-310.
  46. 【79】Anusavic K J. Phillips,(1996),Science of dental materials. 10th edition. Philadelphia: W B Saunders Co,p77~86
  47. 【80】G. J. Tortora,(1999),“Principles of Human Anatomy,” Benjamin/Cummings
  48. 【81】Cameron DA.(1961),The fine structure of osteoblasts in the metaphysis of the tibia of the young rat. J Biophys Biochem Cyto; 9:583.
  49. 【82】Jones SJ, Boyde A. (1977),The migration of osteoblasts. Cell Tissue Res;184,:P179.
  50. 【83】Jotereau FV, LeDouarin NM.(1978) The developmental relationship between osteocytes and osteoclasts:A study using the quail-chick nuclear marker in endochondral ossification. Dev Biol; 63:253.
  51. 【84】Jowsey J,.(1996),Studies of Haversian systems in man and some animals. J Anat; 100:857.
  52. 【85】李政昕,蕭世裕.(2002)Multipotent Stem Cells from Adipose Tissue:Application for PLGA 3D Scaffold in Skeletal Tissue Engineering. 國立成功大學生物科技研究所碩士論文.
  53. 【87】Stein et al. (1996),Transcriptional control of osteoblast growth and differentiation, Physiol. Rev. 76: 593-629
  54. 【88】Raouf A and Seth A; (2002), Transcriptional profiling of human osteoblast differentiation, Journal of Cellular Biochemistry, Volume89,Issue2, P 389 - 400
  55. 【90】董國忠,江彰吉,林峰輝,黃金旺.(2002)A Study of Immobilization of Bone Growth Factor on Calcium PhosphateBioceramics as a Viable Bone substitute.私立中原大學博士論文
  56. 【91】王欣雅,鐘次文,蔡正倫,(2002)”不同生物材料對內皮細胞生長之研究”中原大學醫學工程系碩士論文
  57. 【94】陳桂蘭,江旭禎.(2000)”感應偶合電漿質譜儀於化妝品及奶粉樣品中元素分析之應用校”中山大學化學研究所分析組碩士論文,
  58. 【95】陳俐婷, 張恆雄, 孫瑞昇.(2003) ”Effects of pulse burst electromagnetic fieldstimulation on osteoblasts bioactivities”中原大學醫學工程學系碩士論文,
  59. 【2】長庚紀念醫院 護理部 編印,人工髖關節, www.cgmh.org.tw/health/nursing/ 人工髖關節/人工髖關節.html - 10k -
  60. 【4】劉華昌, (1996) “人工髖關節鬆脫之整體研究:不同物質的顆粒對骨細胞、肌肉細胞與纖維細胞的影響”, 編號NSC 85-2213-E-002-054, 中華民國八十五年國科會醫學工程研究成果發表會論文集, p.21.
  61. 【6】R. L. Williams, S. A. Brown, K. Merritt, Biomaterials, 9, 181, 1988.
  62. 【8】J. M. Haguenoer and D. Furon, “Toxicologie et Hygiene Industrielle, Les Derives Mineraux,” Technique et Documentation, paris
  63. 【11】林奇民, (2000) “電解沉積AI2O3-HA生醫陶瓷於純鈦金屬上之研究”, 國立中興大學 材料工程學系研究所碩士論文
  64. 【12】林素苹, (2001) “The Study of Electrolytic Deposition ZrO2/HA Bioceramic Coatings on Ti-6Al-4V Aalloy”, 國立中興大學 材料工程學系研究所碩士論文
  65. 【13】陳瑞龍,(2004) “電化學沈積HA/TiO2生醫陶瓷鍍層於Ti-6Al-4V合金製
  66. 程參數之研究” 國立中興大學 材料工程學系研究所碩士論文
  67. 【16】Adell R, Lekholm U, Rockler B, Branemark PI. (1981) “A 15-year study of
  68. 【17】Kim, Hae-Won., (2003) “Porous ZrO2 bone scaffold coated with hydroxyapatite
  69. with fluorapatite intermediate layer”, Biomaterials, 24, P.3277-3284
  70. 【18】生物醫用材料,俞耀庭,新文京開發出版有限公司P163∼168
  71. titania buffer layer processed by sol–gel method”, Biomaterials, 25, P.2533-2538
  72. 【21】Yu-Peng Lu, 2004“Plasma-sprayed hydroxyapatite+titania composite bond coat
  73. 【22】Inagaki, M.; Yokogawa, Y.; Kameyama, T.,2003 “Bond strength improvement
  74. of hydroxyapatite/titanium composite coating by partial nitriding during
  75. RF-thermal plasma spraying”, Surface and Coatings Technology, 173, P.1-8
  76. 【23】Jae-Man Choi, Hyoun-Ee Kim and In-Seop Lee, (2000), “Ion-beam-assisted
  77. titaniam buffer layer processed by sol–gel method”, Biomaterials, 25, P.
  78. 2533-2538
  79. 【26】I. Zhitomirsky and A. Petric, (2000)“Cathodic electrodeposition of polymer
  80. films and organoceramic films”, Materials Science and Engineering B, 78, P.
  81. 125-130
  82. 【28】陳威明,“淺談人工關節置換術”, 榮總人第十二卷第十一期
  83. 【29】生醫材料簡介,宋信文、陳松青www.che.nthu.edu.tw/alumnus/2003huikan/i/i4.pdf
  84. 【31】ASTM F-136, “Standard Specification for Wrought Titanium-6 Aluminum-4 Vanadium ELI (Extra Low Interstitial) Alloy for Surgical Implant Applications”, Annual Book or ASTM Standards, American Society for Testing and Materials, Vol. 3.01
  85. 【32】ASTM F-138, “Standard Specification for Wrought 18 Chromium-14 Nickel-2.5 Molybdenum Stainless Steel Bar and Wire for Surgical Implants”, Annual Book or ASTM Standards, American Society for Testing and Materials, Vol. 3.01
  86. 【36】晶星晶體結構專業網,http://www.crystalstars.org/big5/index.html,氧化鈦晶體結構
  87. 【44】F. C. M. Driessens, (1983). "Formation and stability of calcium phosphates in relation to the phase composition of the mineral in calcified tissue", in "Bioceramic of Calcium Phosphate", edited by K. de Groot, pp. 1-32, CRC Press, Inc., BocaRaton, Florida,.
  88. 【45】J. L. Johnson, H. P. Jones and K. V. Rajagopalan, (1997) Journal of Biological Chemistry, 252, P.4994-5003.
  89. 【46】Schindler A,Jeffcoat G L,Pitt C G. 1997, Biodegradable polymer for sustained drug delivery.In :Pearce E M,Schaefgen J R eds. Contemporary Topics in Polymer Science. Vol 2. Plenum, p251~289
  90. 【49】Pitt C G, Schinder A. 1984. Capronor-a biodegradable delivery system for levonor-gestrel. In: Zatachini G L ed. Long-acting Contraceptive Delivery Systems. Philadelphia: Harpen and Row ,64~83
  91. 【50】宋存先,王彭延,孫洪範。2000,聚己內酯體內降解吸收和排泄。生物醫學工程雜誌, 17(1):p25
  92. 【51】徐世昌,(2001.2)生物性高分子-幾丁質與幾丁聚醣之介紹與應用。化工資訊, P36-P45,
  93. 【52】R.A.A.Muzzarelli; R.Rocchetti,(1985)Chitin and Chitosan Fibers, Carbohydrate Polymer, Vol 5,461,
  94. 【58】Minami S, Okamoto T, Matsuhashi A, Sashiwa H, Saimoto H, Shigemasa Y, Tanigawa T, Tanaka Y, Tokura S. 1992. Application of chitin and chitosan in large animal practice. In: Brine CJ, Sand-ford PA, Zikakis JP, editors. Advances in chitin and chitosan. New York: Elsevier Applied Science; p 61–69.
  95. 【59】Borah G, Scott G, Wortham K. 1992. Bone induction by chitosan in endochondral bones of the extremities. In: Brine CJ, Sandford PA, Zikakis JP, editors. Advances in chitin and chitosan. New York: Elsevier Applied Science; p 47–53.
  96. 【60】饒佩如1 廖懿倩1 王姿雅1 許月琴2陳文章1 王怡仁1*1 雲林科技大學化
  97. 工系 2 , 磺化幾丁聚醣在天然氣純化效應之研究,南榮技術學院通識中心,
  98. 計畫編號:NSC 91-2815-C-224-006
  99. 【61】山口壽,(1986),幾丁質與幾丁聚醣在食品與醫藥上之應用,食品開發,21(8):20-23。(Japanese)Publishers,1994,189~212
  100. 【63】陳懿慧、李錦楓,(1996),幾丁聚醣應用於葡萄柚汁澄清之探討,食品科學,23(5):617-628。
  101. 【64】劉瓊淑,1994,幾丁質,幾丁聚醣及相關酵素之特性與應用,食品工業,26(11) : 26-36。
  102. 【65】江晃榮,(1998),生體高分子(幾丁質、膠原蛋白)產業現況與展望
  103. 【66】江晃榮,(1996),新科技產品:幾丁質、幾丁聚醣(甲殼質)產業現況與展望.
  104. 【67】賴惠敏等,(1999),生醫高分子商機與策略
  105. 【68】陳榮輝、金曉珍,水產甲殼類廢棄物開發高經濟價值之幾丁質、幾丁聚醣、幾丁寡醣研究之規劃報導,科學發展月刊,第23卷第6期,p. 550-563.
  106. 【73】田福助,(1993),“電化學 理論與應用”, 臺北巿, 高立出版
  107. 【74】林奇民,顏秀崗,(2002), “Investigation of different Titanium surface treatments on in vitro osteoblast-like cell”, 中國材料科學學會, 2002年材料年會論文集
  108. 【76】Jody Redepenning, Guhanand Venkataraman, Jun Chen, Nathan
  109. Stafford.(2002), Electrochemical preparation of chitosan/hydroxyapatite
  110. composite coatings on titanium substrates. REDEPENNING ET AL.
  111. 【77】http://www.baimtec.com.cn/docc/10_d.htm,人工髖關節
  112. 【78】www.hmbios.com/patients/index products-1-1.html人工髖關節置換手術
  113. Science Publishing, 8th edition, USA.
  114. 【86】楊榮森,“骨質疏鬆症-病因,診斷,治療”,合記圖書出版社,台北,民86
  115. 【89】G.R.Mundy, B.Boyce, D.Hughes, K.Wright, L.Bonewald, S.Dallas,S.Harris, N.Ghosh-Choudhury, D.Chen, C.Dustan, E.Izbicka,T.Yoneda.(1995), The Effect of Cytokines and Growth Factor on Osteoblastic Cells. Bone ; 17(2): 715-755.
  116. 【92】http://wordpedia.pidc.org.tw/Content.asp?ID=37264, 紅外光譜儀, 姚蓓莉
  117. 【93】http://www.wcaslab.com/tech/tbicpms.htm, Metals analysis by ICP-MS
Times Cited
  1. 黃江瑜(2007)。膠原蛋白對已被覆生醫陶瓷Ti6Al4V植入用合金之影響。中興大學材料科學與工程學系所學位論文。2007。1-98。 
  2. 雷震邦(2014)。熱可塑聚氨酯與氫氧基磷灰石複合材料做為人工髖關節的生物相容性與機械性質分析。中山醫學大學醫學研究所學位論文。2014。1-79。