Establishment and Characterization of a Continuous Human Basal Cell Carcinoma Cell Line from Facial Skin (I) Cytological Behavior of Early Passages

能長期培養之人類皮膚基底細胞癌細胞株之建立和特性分析（I）培養初期之細胞考行為

蔣連財(Lien-Chai Chiang) ； 蔣雯(Wen Chiang) ； 余幸司(Hsin-Su Yu) ； 許漢銘(Hamm-Ming Sheu) ； 陳豪勇(Hour-Young Chen)

《The Kaohsiung Journal of Medical Sciences》 10卷4期 (1994/04) Pp. 170-176

https://doi.org/10.6452/KJMS.199404.0170

摘要

皮膚基底細胞癌（basal cell carcinomas, BCCs）為人類最常見之惡性疾病之一。此類癌細胞生長非常慢，其細胞只能夠短期培養，而且染色體大部分為正常之雙套（diploid）。本實驗室近年來所培養之BCCs中，有一病例為臉上燙傷疤所長出之未分化型BCC，其染色體為非整套型（aneuploidy），而且能繼代培養超過100代，將其命名為Bcc-l/KMC細胞株。此細胞株在培養第3代時之細胞群倍增時間為38.86小時。此永恆性細胞株表現有角質蛋白纖維和desmosomes等上皮細胞特徵，而且在裸鼠上會誘生腫瘤。它的遺傳標記為HLA-A2, A24, B27, B35, Cw3, DR2, DR 12。BCC-1/ KMC細胞株已經成功地適應生長於便宜而含有中度鈣離子（0.4 mM）之一般培養基（RPMI 1640）。

並列摘要

Basal cell carcinomas (BCCs) are one of the most common skin malignant diseases of human beings in the course of a lifetime. BCC is a slow growing tumor of epithelial origin. Most of the BCC tumors have a normal diploid DNA configuration and have been successfully passaged for a short-term only. We now report a long-term culture (BCC-1/KMC) of human BCC derived from the undifferentiated type of facial BCC tumor on the thermal traumatic scar, which was aneuploidy and subculture for more than 100 passages. The population doubling time of BCC-1/KMC cells at the third passage was 38.86 hours. This immortalized and tumorigenic cell line expresses epitheial markers of keratin filaments and desmosomes. The genetic markers of this cell line have HLA-A2, A24, B27, B35, Cw3, DR2 and DR12. The BCC-1/KMC cells have successfully adapted to grow in a cheaper commercial medium (RPMI 1640) and at a moderate concentration of calcium (0.4 mM).

並列關鍵字

basal cell carcinoma ； continuous cell line