Intracerebroventricular Ghrelin Enhances Non-Nutrient Semiliquid Gastric Emptying in Fasted Conscious Rats

側腦室投予饑餓激素對空腹清醒大鼠促進非營養性半流體之胃排空效應

陳志彥(Chih-Yen Chen) ； 董明倫(Ming-Luen Doong) ； 賈愛華(Eileen Jea Chien) ； 羅景全(Jiing-Chyuan Luo) ； 盧俊良(Ching-Liang Lu) ； 林漢傑(Han-Chieh Lin) ； 張扶陽(Full-Young Chang) ； 李壽東(Shou-Dong Lee)

《臺灣消化醫學雜誌》 25卷3期 (2008/09) Pp. 242-248

https://doi.org/10.6557/GJT.200809_25(3).0002

摘要

研究動機：雖然饑餓激素(ghrelin)的周邊和中樞攝食效應，和其周邊對消化道蠕動力的影響，已經深入知曉，然而，中樞乙醯化饑餓激素在大鼠對胃之運動力的影響仍未探索。我們首先嘗試建立一個穩定的清醒大鼠模型來測量非營養性半流體胃排空，其次，來探索側腦室注射乙醯化饑餓激素對胃排空的效應。
材料方法：我們探索活性碳非營養性半流體胃排空在長期裝置側腦室導管之十六小時空腹清醒大鼠的時間效應。我們研究在空腹清醒大鼠，側腦室注射乙醯化饑餓激素，對非營養性半流體胃排空的劑量反應性效應。
結果：我們首先建立了在長期置放側腦室導管之清醒大鼠的活性碳非營養性半流體胃排空穩定之時間效應，三十分鐘非營養性半流體胃排空被選爲之後實驗的標的。側腦室注射饑餓激素（0.1, 0.3和1.0nmol）在空腹清醒大鼠增進了三十分鐘非營養性半流體之胃排空。
結論：我們建立了一個可靠的生物體內動物模型，來測量胜肽或化學物質在前大腦影響胃之蠕動力的效應。側腦室投予饑餓激素在空腹清醒大鼠可有效地促進三十分鐘非營養性半流體之胃排空。乙醯化饑餓激素在促進胃排空之最佳化劑量（0.1和0.3 nmol），在未來可選擇爲研究饑餓激素與其它抑制胃腸蠕動力之胜肽的交互作用。

關鍵字

胃排空 ； 饑餓激素 ； 側腦室 ； 蠕動力

並列摘要

Background and Aims: Although peripheral and central impacts of ghrelin on food intake as well as the peripheral influence of ghrelin on gut motility are well known, the central effect of acyl ghrelin on gastric motility in rats remains uninvestigated. We aimed, first, to establish a stable, conscious rat model to measure non-nutrient, semiliquid gastric emptying, and second, to investigate the effect of intracerebroventricular (ICV) injection of acyl ghrelin on gastric emptying.
Methods: We investigated the temporal effects of charcoal, non-nutrient, semiliquid gastric emptying in 16-h food-deprived, conscious rats chronically implanted with ICV cannula. Then, we studied the dose-response effect of ICV acyl ghrelin on charcoal, non-nutrient, semiliquid gastric emptying in fasted conscious rats.
Results: Stable, temporal effects of charcoal, non-nutrient, semiliquid gastric emptying in chronically ICVcannulated, conscious rats were created first, and 30-min non-nutrient, semiliquid gastric emptying was selected among them for the subsequent experiments. ICV-injected ghrelin (0.1, 0.3 and 1.0 nmol/rat) enhanced 30-min nonnutrient, semiliquid gastric emptying in fasted conscious rats.
Conclusions: We established a reliable, in vivo animal model to measure the effects of peptides or chemicals in the forebrain on gastric motility. ICV administration of ghrelin effectively enhances 30-min non-nutrient, semiliquid gastric emptying in fasted conscious rats. The optimal doses of ghrelin at 0.1 and 0.3 nmol on gastric emptying could be chosen in the future to study the interactions between ghrelin and other gut motility inhibitory peptides, such as corticotropin-releasing factor peptide families and cholecystokinin.

並列關鍵字

gastric emptying ； ghrelin ； intracerebroventricular ； motility