利用果蠅評估鎖陽對延緩老化之效果與其作用機制探討

Using Drosophila to study the anti-aging effect of Cynomorium songaricum Rupr. and the mechanisms of its action

張榮富(RongFu Chang)

指導教授 ： 蔡輔仁

亞洲大學/健康學院/生物科技學系碩士班/碩士(2010年)

摘要

老化（Aging），即生物體因年紀增加，其體內系統、器官，以及細胞機能出現衰退等現象，因此尋找增加壽命，達到減少老化疾病發生和探討老化的機轉是當今一大重要的課題。
中藥典籍中鎖陽（Cynomorium songaricum Rupr.）常用來調整內分泌腺、增加人體免疫力的功用。本實驗目的以果蠅模式探討鎖陽是否有延緩老化之效果與可能作用機制。
在本實驗中，我們餵食鎖陽後進行壽命分析，發現母果蠅在29℃時，投予鎖陽的實驗組存活率相對對照組會增加平均壽命11~17%，公果蠅的存活率分析實驗組與對照組是沒有差異。為了探討鎖陽延緩老化的作用機制，我們分析其抗氧化壓力能力，實驗選用投予鎖陽第20天母果蠅進行氧化壓力試驗（如paraquat、H2O2、starvation）、脂質過氧化程度分析、抗氧化酵素活性分析（superoxide dismutase, catalase）、quantitative PCR (qPCR)，以及進行交配能力試驗。在氧化壓力試驗中，投予鎖陽果蠅會減低由paraquat、H2O2、starvation所誘導產生自由基，分別增加其平均壽命9~13%、11~12%、6~12%。脂質過氧化（lipid peroxidation, LPO）分析可知實驗組果蠅體內LPO相對於對照組有減少趨勢；抗氧化酵素活性實驗組有增加趨勢；由qPCR 分析發現實驗組果蠅體內SOD1、SOD2、CAT、Hsp27、IMP-L2、l(2)efl、dilp2、dilp3及dilp5基因表現量均無差異。交配試驗中發現，公果蠅或母果蠅在其交配能力是無差異。結果顯示鎖陽透過提高果蠅體內抗氧化壓力作用，來增加壽命效果，對鎖陽抗老化作用提供科學上佐證。

關鍵字

果蠅 ； 抗氧化 ； 鎖陽

並列摘要

Aging is a process for all organisms and appears degeneration in their cellular and biological functions. Therefore, to study the mechanisms of aging, the ways to extend lifespan, and decline in the incidences of aged-related diseases becomes important issues for scientists. According to the literature of Chinese medicine, Cynomorium songaricum Rupr. is used to improve the functions of kidney, immune system and sexual capability. The purpose of this study is to use Drosophila as an animal model to study the anti-aging effect of C. songaricum Rupr. and explore its possible mechanisms.
In survival analysis, female flies treated with C. songaricum Rupr. were presented the increase in the average lifespan to 11-17% compared with the control group, but there was no significant difference in male flies. In order to study the mechanisms of anti-aging effect, we analyzed the resistance in the response to oxidative stress for flies feeding with C. songaricum Rupr. In the twenty-day-old experimental flies feeding with drug, the average lifespan was increased under oxidative stress analysis, including paraquat, H2O2 and starvation treatment to 9-13%, 11-12% and 6-12%, respectively. We also determined the activity of anti-oxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) and the level of lipid peroxidation (LPO). Our results revealed that the activity of SOD and CAT was increased as well as the level of LPO was decreased in the experimental group compared with the control group. In quantitative PCR (qPCR), there were no expression differences in SOD1, SOD2, CAT, Hsp27, IMP-L2, l(2)efl, dilp2, dilp3 and dilp5 genes. Further, we also analyzed the mating ability after drug treatment, but there was no statistical difference between control and experimental groups. Our results demonstrated that C. songaricum Rupr. could extend lifespan and increase the resistance to oxidative stress, and this may provide a scientific evidence for the anti-aging effect of C. songaricum Rupr.

並列關鍵字

Cynomorium songaricum Rupr ； Drosophila ； AND ； antioxidative

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