- 期刊
海洋深層水原菌與希瓦氏菌雙槽式微生物燃料電池之比較研究
Comparative Assessment of Double-Chamber Microbial Fuel Cells (DC-MFCs) Using Shewanella sp. and Deep-Seawater Microbes
摘要
本研究首次嘗試以海洋深層水為基礎，外加碳、氮、磷源補強之方式來篩選可耐海洋深層水之混合菌，並對海洋深層水混合菌與產電菌Shewanella sp. BC01及先前自行篩選之本土耐鹽菌株在以染料脫色為刺激產電條件下，比較評估應用於微生物燃料電池的可能性。在補強能源基質的條件下，依據海洋深層水和微量元素配方對其進行產電馴化作用，並量化評估生物膜形成的產電與脫色之優劣，再由循環伏安法以測試可能存在之產電中介體及可能機制。在以不同染料濃度、微生物種及培養基的條件下，並對微生物燃料電池的產電性能進行測量，以推論高鹽條件是否可有效減少電池內阻，並提高產電效能，並推論出最佳之可行性產電模式及可能機制。
並列摘要
With energy supplementation of carbon, nitrogen, phosphorus nutrients, several deep sea water (DSW)-bearing mixed cultures were selected as model consortia for strain isolation. We assessed the evolution of electrochemical characteristics of MFC for mixed cultures from intact DSW, bioelectricity-generating strain Shewanella sp. BC01 and indigenous salt-tolerant strains. With the addition of energysources, aforementioned microbes were acclimatized with DSW in different nutrient element ratios. The performances of power-generation and dye decolorization of biofilm cells were also assessed. The cyclic voltammetry measurements were made to determine the mechanisms and potential mediators for current-generation in MFCs. With different concentrations of dyes, bacterial strains and culture media, the performances of MFCs were maintained under high-salt conditions which evidently reduced the internal resistance of MFC and enhanced the performance of current-generation. This study also revealed the most promising mode of current-generation and plausible kinetic mechanisms in MFCs.