Quality Assurance is a growing concern of higher education institutions throughout Pakistan. It is simply because of the emergence of multiple institutions offering similar courses thereby giving plenty of choices to the students. Thus, the choice is now wide and depends mainly on the quality of Education at the institutions. The Higher Education Commission (HEC), Pakistan has also emphasized much on quality assurance measures at higher education institutions in Pakistan. Several measures have been suggested and implemented. This paper presents the impact of introducing one of the quality measures i.e., Teachers Portfolio in one of the institutes of higher education in Karachi, Pakistan. A format for the teacher's portfolio was designed taking into consideration the needs of academic programs, recommendations of the HEC of Pakistan and the practices of some of the international universities. Before introducing the portfolio, the faculty of the selected programs was trained and a coordinator was identified to facilitate the process by evaluating the portfolio in the institution under study as per given guidance. Besides, a checklist was developed and provided to the coordinator for structured feedback on portfolio. Finally, peer evaluation technique was used for the subjective evaluation of the quality of the portfolio. The impact has been studied from portfolio reports and feedback from teachers and students. The remarkable results show a marked improvement in teaching and learning. Secondly, effective reporting from the coordinator energized the faculty members and resulted in improving their interaction, teaching methodology and lecture organization. After the peer evaluation, the faculty members revised and maintained their portfolio incorporating the necessary changes as suggested in the feedback and showed a high level of satisfaction on this healthy exercise which created a healthy competition among them.