The Skin Irritation and Pharmacology of Cinnamic Aldehyde in Sport-Injury Medication

運動傷害用藥中桂皮醛的皮膚刺激性與藥理

徐欽賢(Chin-Hsien Hsu) ； 廖俊旺(Jiunn-Wang Liao) ； 陳福安(Fu-An Chen)

《Annual Journal of Physical Education and Sports Science》 2期 (2002/12) Pp. 65-72

https://doi.org/10.6694/JPESS.200212_(2).0006

摘要

維生素B12是蛋白質合成的觸媒之一，且在能量代謝上扮演著重要的角色，部分優秀運動員亦經常補充維生素B12；而良好品質的維生素B12的是補充時需注意的條件之一，因此建立一個良好的分析條件是很重要的。本研究的目的在於探討一個適當的高效液相色層分析(HPLC)條件，將維生素B12(Cyanocobalamin)與其他的水溶性維生素分離，以檢測藥品中之維生素B12的含量。實驗以Alltima C18的管柱、磷酸二氫鉀與乙腈溶液為移動相、結果顯示以磷酸二氫鉀與乙腈溶液（860:140，V／V）於1.0ml／min的流速下可將維生素B12與其他之水溶性維生素分離，且於210nm的紫外線吸收光譜下維生素B12有較大的吸收峰。又以cephazolin作為內標準品，維生素B12與cephazolin之吸收光譜出現的時間分別約為8.9及7.7分鐘。維生素B12的濃度為0.15-0.75μg／ml時吸收光譜中維生素B12與cephazolin波峰高度的比值成一線性相關（r=O.999）。市售商品標準添加之回收率大於96％。此外，將含有維生素B12的原料藥－市售膠囊、針劑及膜衣錠，以所發展的HPLC法與美國藥典微生物分析法定量維生素B12的含量，並以paired t-test做一配對比較，結果顯示兩方法間並無顯著差異。此結果顯示本分析方法可代替美國藥典之微生物分析法來定量藥物中維生素B12的含量，且更為快速、穩定並具再現性。此分析方法可用於檢測市售維生素B12製劑，以協助運動員選擇品質良好的維生素B12，才能達到實際補充的效用。

關鍵字

皮膚刺激性 ； 非類固醇消炎藥 ； 桂皮醛 ； 運動傷害

並列摘要

Vitamin B12,one of the vitamin B complex,plays an important role inenergy metabolism and also acts as catalysts in protein synthesis.Some eliteathletes also supply vitamin B12 as a nutritional ergogenic aid.Since the goodquality is essential,an analyze method to determine the amount of vitamin B12 inpharmaceutical preparations is necessary.A rapid,simple,and reliablereversed-phase high performance liquid chromatographic(HPLC)method wasdeveloped for the assay of cyanocobalamin(Vit B12)in bulk drug andpharmaceutical preparations.Cyanocobalamin and other water-soluble vitaminscan be also separated.The samples was analyzed on an Alltima C18(25 cm×4.6mm I.D.;particle size,5μm)column with a mobile phase of 25 mM potassiumdihydrogen phosphate/acetonitile(V/V=86/14)at a flow-rate of 1.0ml/minand detection at 210nm.Cephazolin was used as an internal standard.Theretention times of cyanocobalamin and cephazolin were 8.9 and 7.7 minutes.Anequation was presented for linear relationship between peak height ratios ofcyanocobalamin to cephazolin and the cyanocobalamin concentration over arange of 0.15-0.75μg/ml(r=0.999).Standard addition recoveries were greaterthan 96% with four pharmaceutical preparations.Furthermore,paired assayresults by microbiological medium diffusion assay and described HPLC methodswere obtained from bulk drug,500μg and 100μg capsule,62.5μg tablet,1000μg injection,containing cyanocobalamin.Statistical tests were used to assess theequivalence of the paired assay results.At the 95% confidence level,nosignificant intermethod differences were noted for the assay results for each pair.The results indicated the proposed HPLC method is suitable substitute for themicrobiological method of the United States Pharmacopeia for potency assays ofcyanocobalamin preparations.The analytical method can be used to determinethe commercial vitamin B12 preparations.It can help athletes to choose the goodquality of vitamin B12 and achieve the best supplementation.

並列關鍵字

Skin Irritation ； Non-Steroidal Anti-Inflammatory Drug ； Cinnamic Aldehyde ； Sport-Injury Medication