防己藥材中馬兜鈴酸分析方法之建立與含量調查

Determination of Aristolochic Acid in Fangchi Radix

徐雅慧(Ya-Hue Hsu) ； 曾信雄(Hshinn-Hshiung Tseng) ； 溫國慶(Kuo-Ching Wen)

《藥物食品檢驗局調查研究年報》 15號 (1997/09) Pp. 136-142

https://doi.org/10.6946/ASRNL.199709.0136

摘要

以高效液相層析儀，分析防己中馬兜鈴酸Ⅰ、Ⅱ (aristolochic acid Ⅰ、Ⅱ)之含量。採用C18逆相層析管柱，移動相為乙腈-1.25%醋酸溶液(40:60)，檢測波長為254 nm，得到良好之分析結果。馬兜鈴酸Ⅰ、Ⅱ在濃度0.63~40.00μg/mL、0.40~25.76μg/mL範圍內，利用上述分析技術進行對照標準品馬兜鈴酸Ⅰ、Ⅱ之分析得線性迴歸方程式及相關係數(r)分別為Y=1.06E-05X-0.28(r=0.9998)，Y=8.28E-06X+0.12(r=0.9998)，顯示良好線性關係。馬兜鈴酸Ⅰ之同日內及異日間之相對標準偏差為1.17%及1.71%，添加回收率為103.08±3.42%，馬兜鈴酸Ⅱ之同日內及異日間之相對標準偏差為1.28%及1.70%，添加回收率為103.13±3.68%。價購市售防己藥材二十件，利用上述高效液相層析法分析，、馬兜鈴酸Ⅰ含量介於0.030~0.192%之間，平均含量為0.086%，馬兜鈴酸Ⅱ含量介於0.017~0.118‰間，平均含量為0.043‰。

關鍵字

馬兜鈴酸Ⅰ ； Ⅱ aristolochic acidⅠ、Ⅱ ； 高效液相層析法HPLC ； 含量測定

並列摘要

A high performance liquid chromatographic method for determination of aristolochic acid il and Ⅱ in Fangchi Radix was established. The samples were analyzed by HPLC on a Cosmosil 5C18-AR column and UV detector (254 nm) with acetonitrile-1.25% acetic acid (v/v) (40:60) as a mobile phase at a flow rate of. 1.0 mL/min. The regression equations of aristolochic acid Ⅰ and Ⅱ were Y = 1.06E-05X-0.28 (r=0.9998) and Y = 8.28E-06X+0.12 (r=0.9998), respectively. The intraday and interday relative standard deviations of aristolochic acid Ⅰ were 1.17% and 1.71%, and the recovery was 103.08±3.42%. The intraday and interday relative standard deviations of aristolochic acid Ⅱ were 1.28% and 1.70%, and the recovery was 103.13±3.68%. 20 samples of Fangchi Radix were analyzed. Results showed that average contents of aristolochic acid Ⅰ and Ⅱ in samples were 0.086% and 0.043‰, and the ranges.