Analysis of Rhubarb by Liquid Chromatography-Electrospray-Mass Spectrometry

Ming-Ren S. Fuh ； Hung-Jian Lin

《淡江理工學刊》 6卷1期 (2003/03) Pp. 31-36

https://doi.org/10.6180/jase.2003.6.1.04

並列摘要

Liquid chromatography-electrospray-mass spectrometry (LC-ES-MS) was employed to analyze five biologically active components (sennoside A and B, rhein, emodin and aloe-emodin) in rhubarb. The separation was achieved on a C18 column using gradient elution. Negative ion detection mode was utilized for ES-MS detection. For sennosides, [M-H](superscript -) and a fragmented ion (m/z=386) were detected. In addition to [M-H](superscript -), [M-COOH](superscript -) was observed for rhein. Only one major ion, [M-H](superscript -), was determined for aloe-emodin and emodin. A post-column splitter was employed to enhance the sensitivity of sennoside measurement. Extracted ion monitoring was applied for quantitative analysis. Satisfactory linearity (r^2=0.99) of each compound was determined. In addition, the content of these five active components in various rhubarb samples were examined by this newly developed LC-ES-MS.

並列關鍵字

LC-MS ； Chinese Herbal Medicine ； Sennoside