In this study, ten whey samples collected from dairy industries in Rasht (Iran). Five lactose fermentative yeasts strains (designated A1 to A5) were isolated. Beta-galactosidase activity in the yeast strains showed that strain of Kluyveromyces marxianus designated as A2 had highest enzyme activity (up to 9012 EU/mL) and the most SCP production from whey with the yield of 12.68 g/L. Ammonium sulfate as nitrogen source had an increasing effect on biomass yield up to 30%. Crude fiber, lipids, carbohydrates and ash content of isolate dry cells were found to be 4.9, 7.23, 33.19 and 14.05%, respectively. The true protein content based on nitrogen fractionation procedure was 29.25%. The yeast biomass recovering by ultrafiltration reduced the total COD to 96.26% of its initial value in the raw whey.
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