拓印聚乙烯乙烯醇/氧化鋅柱電化學感測電極製備及其膀胱癌人類檢體試驗

人體尿液中被發現核基質蛋白22(NMP-22)、3-羥基鄰氨基苯甲酸(3-HAA)及犬尿酸(KYNA)為檢測膀胱癌之指標分子。本研究中以水熱法於金電極表面成長奈米氧化鋅柱，其直徑約為265.31 ± 39.91 nm，並以溶劑揮發法製備出NMP-22、3-HAA及KYNA分子拓印聚乙烯乙烯醇於氧化鋅電極，以可攜式恆電位儀進行非侵入式的電化學檢測。NMP-22對於乙烯比例為27 mole%的聚乙烯乙烯醇有較佳的拓印效率值。以NMP-22拓印氧化鋅電極對比NMP-22拓印電極，在吸附濃度100 pg/mL時，拓印氧化鋅電極之電流差值為64.56 ± 3.46 uA/cm2，而拓印薄膜電極之電流差值為37.13 ± 2.16 uA/cm2，差異可達1.74倍；以3-HAA對拓印氧化鋅電極對比3-HAA拓印電極，在吸附濃度10 pg/mL時，拓印氧化鋅電極之電流差值為34.98 ± 0.63 uA/cm2，而拓印薄膜電極之電流差值為23.21 ± 1.00 uA/cm2，差異可達1.51倍；以KYNA對拓印氧化鋅電極對比KYNA拓印電極，在吸附濃度100 pg/mL時，拓印氧化鋅電極之電流差值為29.66 ± 1.24 uA/cm2，而拓印薄膜電極之電流差值為18.82 ± 2.02 uA/cm2，差異可高達1.58倍，證明成長氧化鋅確實能提升靈敏度。進行電化學干擾性測試，可得知三種分子拓印氧化鋅電極對於干擾因子影響程度低。以循環伏安法進行添加法量測檢體尿液，結果得知準確率最高可達95.53 ± 3.35 %。本研究與成大醫院泌尿科醫生合作並進行人體臨床試驗，利用NMP22、3-HAA拓印奈米氧化鋅電極結合注射式微流體系統進行尿液檢體量測，其檢測膀胱癌之準確率分別為75.0%和87.5%。

關鍵字

核基質蛋白22 ； 3-羥基鄰氨基苯甲酸；犬尿酸；尿液；膀胱癌；分子拓印；氧化鋅；聚乙烯乙烯醇；人體臨床試驗

並列摘要

Nuclear matrix protein no.22(NMP22), 3-Hydroxyanthranilic acid(3-HAA) and kynurenic acid(KYNA) were found in patients’ urine of with bladder cancer. In this work, Zinc oxide(ZnO) rods were hydrothermally grown on a gold substrate; the rods had an average length and diameter of 1.08±0.11 μm and 265.31±39.91 nm, respectively. NMP-22, 3-HAA and KYNA were imprinted into poly(ethylene-co-vinyl alcohol), EVAL, which were coated onto ZnO rod arrays. Using non-invasive electrochemical detection in urine samples by portable potentiostat. The higher imprinting effectiveness for NMP-22 was obtained with 27 mole % of ethylene. The electrochemical response with MIPs/ZnO electrodes was 1.5-fold higher than that with MIPs by the injection microfluidic CV system. In human clinical trials, the sensitivity of NMP-22 and 3-HAA were 75.0 and 87.5%.

並列關鍵字

Nuclear matrix protein no.22 ； 3-hydroxyanthranilic acid ； kynurenic acid ； bladder cancer ； urine ； poly(ethylene-co-vinyl alcohol)(EVAL) ； Zinc Oxide ； human clinical trials

參考文獻

[6] Vinay Kumar, Abul K. Abbas, Nelson Fausto and Jon Aster, Robbins & Cotran Pathologic Basis of Disease, 8th Edition, 2010.

[8] M.H. Kanitz, S. Swaminathan and R.E. Savage Jr, “Two-dimensional electrophoretic protein profile analysis following exposure of human uroepithelial cells to occupational bladder carcinogens”, Cancer Letters, vol.205, 2004, pp.121-131.

[9] K.K. H. Aben and L.A.L. M. Kiemeney, “Epidemiology of Bladder Cancer”, European Urology, vol.36, 1999, pp.660-672.

[10] K.-H. Shen, C.-C. Wu, Y.-H. Wang, “Sulfotransferase 1A1 G638A polymorphism, cigarette smoking and bladder cancer risk in Taiwan”, Advances in Bioscience and Biotechnology, vol.03, 2012, pp.186-190.

[11] W.J. Vermaak, J.B. Ubbink, H.C. Barnard, G.M. Potgieter, H. van Jaarsveld and A.J. Groenewald, “Vitamin B-6 nutrition status and cigarette smoking” Am J Clin Nutr, vol.51, 1990, pp.1058-1061.

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