Green fluorescent protein (GFP) from Aequorea victoria is a novel marker compared with other bioassay markers that has potential use in the study of teleost development biology, transgenic research, and aquaculture. Microinjection and particle gun were used to transfer pCMV-β into fertilized zebra fish eggs. After staining analysis, the data showed80% and 6% expression rates, respectively. After microinjection of the pEGFP-C1 vector in to fertilized zebra fish eggs, the expression rate was 80%.GFP was visible 5 h after micro injection of the pEGFP-C1 vector. Following advancement of the development stage, the signal was visible in muscle skeletal fibers, heart, and corpuscles of living embryos. Transgenic fish continued to express high levels of GFP in bone marrow, eyes, and muscle in 42-d-old zebra fish as revealed by histological sections data.